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Literature summary for 1.10.3.2 extracted from

  • Mancini, S.; Kumar, R.; Mishra, V.; Solioz, M.
    Desulfovibrio DA2_CueO is a novel multicopper oxidase with cuprous, ferrous and phenol oxidase activity (2017), Microbiology, 163, 1229-1236 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Desulfovibrio sp. A2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) pH 7.0, 25°C Desulfovibrio sp. A2

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Desulfovibrio sp. A2
-
-

Organism

Organism UniProt Comment Textmining
Desulfovibrio sp. A2 G2H480 bifunctional copper oxidase and laccase, cf. EC 1.16.3.4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, by Ni-NTA affinity chromatography Desulfovibrio sp. A2

Renatured (Commentary)

Renatured (Comment) Organism
the isolated enzyme does not exhibit any enzymatic activity and lacks the blue colour typical of multicopper oxidase. Supplementation of purified CueO with CuSO4 activates the enzyme and renders it blue Desulfovibrio sp. A2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Desulfovibrio sp. A2 ?
-
 ?
2,3-dihydroxybenzoate + H2O
-
 Desulfovibrio sp. A2 ?
-
 ?
2,6-dimethoxyphenol + H2O
-
 Desulfovibrio sp. A2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 71000, calculated from sequence Desulfovibrio sp. A2

Synonyms

Synonyms Comment Organism
CueO
-
 Desulfovibrio sp. A2
DA2_0547 locus name Desulfovibrio sp. A2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 2,3-Dihydroxybenzoate pH 7.0, 25°C Desulfovibrio sp. A2
0.58
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) pH 7.0, 25°C Desulfovibrio sp. A2

pH Range

pH Minimum pH Maximum Comment Organism
5.1
-
 no activity with substrate [Cu(I)(bicinchoninate)2]3- Desulfovibrio sp. A2

General Information

General Information Comment Organism
physiological function when expressed in an Escherichia coli CueO knock-out strain, enzyme exhibits phenol oxidase activity in vivo and enhances the copper tolerance of the strain Desulfovibrio sp. A2