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Literature summary for 1.10.3.2 extracted from

  • Berini, F.; Verce, M.; Ausec, L.; Rosini, E.; Tonin, F.; Pollegioni, L.; Mandic-Mulec, I.
    Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens (2018), Appl. Microbiol. Biotechnol., 102, 2425-2439 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged enzyme is heterologously expressed in Escherichia coli Geobacter metallireducens

Inhibitors

Inhibitors Comment Organism Structure
2,6-dimethoxyphenol substrate inhibition Geobacter metallireducens
DMSO 10%, 50% loss of the original activity Geobacter metallireducens
Tween-80 1%, more than 50% loss of activity Geobacter metallireducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 O2 25°C, pH 5.6 Geobacter metallireducens
0.62
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 25°C, pH 5.6 Geobacter metallireducens
5.1
-
 2,6-dimethoxyphenol 25°C, pH 5.6 Geobacter metallireducens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Geobacter metallireducens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper multicopper oxidase. The apoenzyme is inactive. Incubation with CuSO4 allows a 43fold increase of the specific activity yielding a metallo-enzyme Geobacter metallireducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52700
-
 gel filtration Geobacter metallireducens

Organism

Organism UniProt Comment Textmining
Geobacter metallireducens Q39TP1
-
-
Geobacter metallireducens GS-15 Q39TP1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacter metallireducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Geobacter metallireducens ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Geobacter metallireducens ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
guaiacol + O2
-
 Geobacter metallireducens ?
-
 ?
guaiacol + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
K4[Fe(CN)6] + O2
-
 Geobacter metallireducens ?
-
 ?
pyrogallol + O2
-
 Geobacter metallireducens ?
-
 ?
pyrogallol + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
syringaldazine + O2
-
 Geobacter metallireducens ?
-
 ?
syringaldazine + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?

Synonyms

Synonyms Comment Organism
GMET_RS10855
-
 Geobacter metallireducens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Geobacter metallireducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol Geobacter metallireducens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 good stability in the pH range 5.0-6.0 Geobacter metallireducens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
95.4
-
 2,6-dimethoxyphenol 25°C, pH 5.6 Geobacter metallireducens