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Literature summary for 1.10.3.2 extracted from

  • Wang, H.; Huang, L.; Li, Y.; Ma, J.; Wang, S.; Zhang, Y.; Ge, X.; Wang, N.; Lu, F.; Liu, Y.
    Characterization and application of a novel laccase derived from Bacillus amyloliquefaciens (2020), Int. J. Biol. Macromol., 150, 982-990 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Application

Application Comment Organism
environmental protection the enzyme is an ideal candidate for lots of biotechnological and industrial applications due to its stability in the extreme conditions Bacillus amyloliquefaciens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.1 mM, 65.5% inhibition Bacillus amyloliquefaciens
BaCl2 5 mM, 10% inhibition Bacillus amyloliquefaciens
CoCl2 5 mM, 19% inhibition Bacillus amyloliquefaciens
DTT 0.1 mM, complete inhibition Bacillus amyloliquefaciens
EDTA 5 mM, 51.5% inhibition Bacillus amyloliquefaciens
FeCl3 5 mM, 14% loss of activity Bacillus amyloliquefaciens
FeSO4 5 mM, complete loss of activity Bacillus amyloliquefaciens
L-cysteine 0.5 mM, 67% inhibition Bacillus amyloliquefaciens
MnCl2 5 mM, 90% loss of activity Bacillus amyloliquefaciens
NaCl 10 mM, 20% inhibition. 500 mM, 63% inhibition. 1000 mM, complete loss of activity Bacillus amyloliquefaciens
NiSO4 5 mM, 4% inhibition Bacillus amyloliquefaciens
SDS 5 mM, 17% inhibition Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
copper copper-containing enzyme Bacillus amyloliquefaciens
MgCl2 0.5 mM, 1.08fold activation Bacillus amyloliquefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 calculated from sequence, SDS-PAGE Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens A0A6C0GY11
-
-
Bacillus amyloliquefaciens TCCC 111018 A0A6C0GY11
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus amyloliquefaciens ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Bacillus amyloliquefaciens ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
Adizol black B + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
Azophloxine + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
Congo Red + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
Crystal violet + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
indigo carmine + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
indigo carmine + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
Malachite Green + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
Malachite Green + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
Reactive blue 19 + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
Reactive blue 5 + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
Remazol Brilliant Blue R + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
Remazol Brilliant Blue R + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?

Synonyms

Synonyms Comment Organism
CotA-laccase
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to when using the substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens
7
-
 substrate: 2,6-dimethoxyphenol Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about 35% of maximal activity, pH 7.0: about 55% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens
6 9 pH 6.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity, substrate: 2,6-dimethoxyphenol Bacillus amyloliquefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 stable up to when using the substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens