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Literature summary for 1.10.3.2 extracted from

  • Kolyadenko, I.; Scherbakova, A.; Kovalev, K.; Gabdulkhakov, A.; Tishchenko, S.
    Engineering the catalytic properties of two-domain laccase from Streptomyces griseoflavus Ac-993 (2021), Int. J. Mol. Sci., 23, 65 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.2

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method Streptomyces griseoflavus

Protein Variants

Protein Variants Comment Organism
H165A/M199G 2.3fold increase in kcat/Km towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Streptomyces griseoflavus
H165A/R240H 1.8 fold increase in kcat/Km towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Streptomyces griseoflavus
H165A/R240H 4.8fold increase in kcat/Km for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). 15.1fold increase in kcat/Km for 2,6-dimethoxyphenol. 1.4fold decrease in kcat/Km for K4[Fe(CN)6] Streptomyces griseoflavus
M199A 2.4fold increase in kcat/Km for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). 1.4fold increase in kcat/Km for 2,6-dimethoxyphenol. 2.5fold decrease in kcat/Km for K4[Fe(CN)6] Streptomyces griseoflavus
M199A 2.4fold increase in kcat/Km towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Streptomyces griseoflavus
M199G 5.4fold increase in kcat/Km for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). 4.7fold increase in kcat/Km for 2,6-dimethoxyphenol. 1.5fold decrease in kcat/Km for K4[Fe(CN)6] Streptomyces griseoflavus
M199G 5.4fold increase in kcat/Km towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Streptomyces griseoflavus
M199G/H165A 2.3fold increase in kcat/Km for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). kcat/Km for 2,6-dimethoxyphenol is identical to kcat/Km of wilde-type enzyme. 2.7fold decrease in kcat/Km for K4[Fe(CN)6]. Drastic shift in the optimal pH of 2,6-dimethoxyphenol oxidation Streptomyces griseoflavus
M199G/R240H 1.8fold increase in kcat/Km for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). 1.5fold decrease in kcat/Km for 2,6-dimethoxyphenol. 2.4fold decrease in kcat/Km for K4[Fe(CN)6]. Combination of substitutions in the substrate-binding pocket and in the tunnel leading to Cu2+ ion increases the catalytic activity towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) by 5fold, and towards 2,6-dimethoxyphenol by 16fold Streptomyces griseoflavus
M199G/R240H mutation increases catalytic activity of the enzyme towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) by 5fold, and towards 2,6-dimethoxyphenol by 16fold. Prospective 2D laccase variant with the highest activity under alkaline conditions Streptomyces griseoflavus
Y230A 4.02fold increase in kcat/Km for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). 2fold increase in kcat/Km for 2,6-dimethoxyphenol. 1.4fold decrease in kcat/Km for K4[Fe(CN)6] Streptomyces griseoflavus
Y230A 4.0fold increase in kcat/Km towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Streptomyces griseoflavus

Inhibitors

Inhibitors Comment Organism Structure
NaN3 10 mM, residual activity of wild-type enzyme is 23%, residual activity of mutant enzyme M199A is 13%, residual activity of mutant enzyme M199G is 20%, residual activity of mutant enzyme Y230A is 20%, residual activity of mutant enzyme H165A/M199G is 1116%, residual activity of mutant enzyme H165A/R240H is 78%, residual activity of mutant enzyme M199G/R240H is 25% Streptomyces griseoflavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 2,6-dimethoxyphenol wild-type enzyme, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.075
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme H165A/R240H Streptomyces griseoflavus
0.075
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H165A/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.09
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme H165A/M199G Streptomyces griseoflavus
0.09
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H165A/M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme M199G/R240H Streptomyces griseoflavus
0.1
-
 K4[Fe(CN)6] 30°C, pH 4.0, wild-type enzyme Streptomyces griseoflavus
0.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199G/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.15
-
 2,6-dimethoxyphenol 30°C, pH 9.0, mutant enzyme H165A/R240H Streptomyces griseoflavus
0.15
-
 2,6-dimethoxyphenol mutant enzyme H165A/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.17
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme Y230A Streptomyces griseoflavus
0.17
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199G/R240H Streptomyces griseoflavus
0.17
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y230A, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.18
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme H165A/R240H Streptomyces griseoflavus
0.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, mutant enzyme M199G Streptomyces griseoflavus
0.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.24
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme H165A/M199G Streptomyces griseoflavus
0.25
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199G Streptomyces griseoflavus
0.25
-
 2,6-dimethoxyphenol 30°C, pH 8.5, mutant enzyme M199G/R240H Streptomyces griseoflavus
0.25
-
 2,6-dimethoxyphenol mutant enzyme M199G/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.28
-
 2,6-dimethoxyphenol 30°C, pH 6.5, mutant enzyme H165A/M199G Streptomyces griseoflavus
0.28
-
 2,6-dimethoxyphenol mutant enzyme H165A/M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.31
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme Y230A Streptomyces griseoflavus
0.32
-
 2,6-dimethoxyphenol 30°C, pH 9.0, wild-type enzyme Streptomyces griseoflavus
0.32
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, mutant enzyme M199A Streptomyces griseoflavus
0.32
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199A, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.35
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199A Streptomyces griseoflavus
0.36
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type enzyme, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.36
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, wild-type enzyme Streptomyces griseoflavus
0.67
-
 2,6-dimethoxyphenol 30°C, pH 8.0, mutant enzyme M199G Streptomyces griseoflavus
0.67
-
 2,6-dimethoxyphenol mutant enzyme M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.76
-
 2,6-dimethoxyphenol 30°C, pH 8.5, mutant enzyme Y230A Streptomyces griseoflavus
0.76
-
 2,6-dimethoxyphenol mutant enzyme Y230A, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.83
-
 2,6-dimethoxyphenol 30°C, pH 8.0, mutant enzyme M199A Streptomyces griseoflavus
0.83
-
 2,6-dimethoxyphenol mutant enzyme M199A, 30°C, pH not specified in the publication Streptomyces griseoflavus

Organism

Organism UniProt Comment Textmining
Streptomyces griseoflavus A0A0M4FJ81
-
-
Streptomyces griseoflavus Ac-993 A0A0M4FJ81
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces griseoflavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Streptomyces griseoflavus ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Streptomyces griseoflavus Ac-993 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Streptomyces griseoflavus ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Streptomyces griseoflavus Ac-993 ?
-
 ?
K4[Fe(CN)6] + O2
-
 Streptomyces griseoflavus ?
-
 ?
K4[Fe(CN)6] + O2
-
 Streptomyces griseoflavus Ac-993 ?
-
 ?
Malachite green
-
 Streptomyces griseoflavus indigo carmine
-
 ?
Malachite green
-
 Streptomyces griseoflavus Ac-993 indigo carmine
-
 ?

Synonyms

Synonyms Comment Organism
two-domain laccase
-
 Streptomyces griseoflavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzyme M199G Streptomyces griseoflavus
70
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzymes M199A and Y230A Streptomyces griseoflavus
80
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), wild-type enzyme Streptomyces griseoflavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 30 min, wild-type enzyme shows 60% of initial activity after incubation, mutant enzymes M199G and Y230A exhibit less than 30% of the initial activity Streptomyces griseoflavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 2,6-dimethoxyphenol 30°C, pH 9.0, mutant enzyme H165A/R240H Streptomyces griseoflavus
0.11
-
 2,6-dimethoxyphenol mutant enzyme H165A/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.31
-
 2,6-dimethoxyphenol 30°C, pH 6.5, mutant enzyme H165A/M199G Streptomyces griseoflavus
0.31
-
 2,6-dimethoxyphenol mutant enzyme H165A/M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.35
-
 2,6-dimethoxyphenol wild-type enzyme, 30°C, pH not specified in the publication Streptomyces griseoflavus
0.35
-
 2,6-dimethoxyphenol 30°C, pH 9.0, wild-type enzyme Streptomyces griseoflavus
1.3
-
 2,6-dimethoxyphenol 30°C, pH 8.0, mutant enzyme M199A Streptomyces griseoflavus
1.3
-
 2,6-dimethoxyphenol mutant enzyme M199A, 30°C, pH not specified in the publication Streptomyces griseoflavus
1.68
-
 2,6-dimethoxyphenol 30°C, pH 8.5, mutant enzyme Y230A Streptomyces griseoflavus
1.68
-
 2,6-dimethoxyphenol mutant enzyme Y230A, 30°C, pH not specified in the publication Streptomyces griseoflavus
3.39
-
 2,6-dimethoxyphenol 30°C, pH 8.0, mutant enzyme M199G Streptomyces griseoflavus
3.39
-
 2,6-dimethoxyphenol mutant enzyme M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
4.18
-
 2,6-dimethoxyphenol 30°C, pH 8.5, mutant enzyme M199G/R240H Streptomyces griseoflavus
4.18
-
 2,6-dimethoxyphenol mutant enzyme M199G/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme H165A/R240H Streptomyces griseoflavus
6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H165A/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme H165A/M199G Streptomyces griseoflavus
9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H165A/M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type enzyme, 30°C, pH not specified in the publication Streptomyces griseoflavus
15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, wild-type enzyme Streptomyces griseoflavus
20
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme M199G/R240H Streptomyces griseoflavus
20
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199G/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
29
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme Y230A Streptomyces griseoflavus
29
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme H165A/R240H Streptomyces griseoflavus
29
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y230A, 30°C, pH not specified in the publication Streptomyces griseoflavus
33
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, mutant enzyme M199A Streptomyces griseoflavus
33
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199A, 30°C, pH not specified in the publication Streptomyces griseoflavus
35
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme H165A/M199G Streptomyces griseoflavus
38
-
 K4[Fe(CN)6] 30°C, pH 4.0, wild-type enzyme Streptomyces griseoflavus
45
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, mutant enzyme M199G Streptomyces griseoflavus
45
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
48
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199G/R240H Streptomyces griseoflavus
54
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199A Streptomyces griseoflavus
66
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199G Streptomyces griseoflavus
86
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme Y230A Streptomyces griseoflavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzyme H165A/M199G, mutant enzyme M199G/R240H, mutant enzyme H165A/R240H Streptomyces griseoflavus
3.5
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzymes Y230A, H165A/M199G, M199G/R240H and H165A/R240H Streptomyces griseoflavus
4
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), wild-type enzyme, mutant enzyme M199A and mutant enzyme M199G. Substrate: K4[Fe(CN)6], wil-type enzyme, and mutant enzymes M199A, M199G, Y230A, H165A/M199G, M199G/R240H and H165A/R240H Streptomyces griseoflavus
4
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), wild-type enzyme, mutant enzyme M199A, mutant enzyme M199G and mutant enzyme Y230A Streptomyces griseoflavus
6.5
-
 substrate: 2,6-dimethoxyphenol, mutant enzyme H165A/M199G Streptomyces griseoflavus
8
-
 substrate: 2,6-dimethoxyphenol, mutant enzyme M199G and mutant enzyme M199A Streptomyces griseoflavus
8
-
 substrate: 2,6-dimethoxyphenol, mutant enzymes M199A and M199G Streptomyces griseoflavus
8.5
-
 substrate: 2,6-dimethoxyphenol, mutant enzyme Y230A and M199G/R240H Streptomyces griseoflavus
8.5
-
 substrate: 2,6-dimethoxyphenol, mutant enzyme Y230A and mutant enzyme M199G/R240H, Streptomyces griseoflavus
9
-
 substrate: 2,6-dimethoxyphenol, wild-type enzyme and mutant enzyme H165A/R240H Streptomyces griseoflavus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.74
-
 2,6-dimethoxyphenol 30°C, pH 9.0, mutant enzyme H165A/R240H Streptomyces griseoflavus
0.74
-
 2,6-dimethoxyphenol mutant enzyme H165A/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
1.09
-
 2,6-dimethoxyphenol wild-type enzyme, 30°C, pH not specified in the publication Streptomyces griseoflavus
1.09
-
 2,6-dimethoxyphenol 30°C, pH 9.0, wild-type enzyme Streptomyces griseoflavus
1.11
-
 2,6-dimethoxyphenol 30°C, pH 6.5, mutant enzyme H165A/M199G Streptomyces griseoflavus
1.11
-
 2,6-dimethoxyphenol mutant enzyme H165A/M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
1.55
-
 2,6-dimethoxyphenol 30°C, pH 8.0, mutant enzyme M199A Streptomyces griseoflavus
1.55
-
 2,6-dimethoxyphenol mutant enzyme M199A, 30°C, pH not specified in the publication Streptomyces griseoflavus
2.22
-
 2,6-dimethoxyphenol 30°C, pH 8.5, mutant enzyme Y230A Streptomyces griseoflavus
2.22
-
 2,6-dimethoxyphenol mutant enzyme Y230A, 30°C, pH not specified in the publication Streptomyces griseoflavus
5.07
-
 2,6-dimethoxyphenol 30°C, pH 8.0, mutant enzyme M199G Streptomyces griseoflavus
5.07
-
 2,6-dimethoxyphenol mutant enzyme M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
16.51
-
 2,6-dimethoxyphenol 30°C, pH 8.5, mutant enzyme M199G/R240H Streptomyces griseoflavus
16.51
-
 2,6-dimethoxyphenol mutant enzyme M199G/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
42
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type enzyme, 30°C, pH not specified in the publication Streptomyces griseoflavus
42
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, wild-type enzyme Streptomyces griseoflavus
74
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme H165A/R240H Streptomyces griseoflavus
74
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H165A/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
95
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme H165A/M199G Streptomyces griseoflavus
95
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H165A/M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
102
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, mutant enzyme M199A Streptomyces griseoflavus
102
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199A, 30°C, pH not specified in the publication Streptomyces griseoflavus
144
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme H165A/M199G Streptomyces griseoflavus
155
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199A Streptomyces griseoflavus
160
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme H165A/R240H Streptomyces griseoflavus
169
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme Y230A Streptomyces griseoflavus
169
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y230A, 30°C, pH not specified in the publication Streptomyces griseoflavus
203
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.5, mutant enzyme M199G/R240H Streptomyces griseoflavus
203
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199G/R240H, 30°C, pH not specified in the publication Streptomyces griseoflavus
226
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 4.0, mutant enzyme M199G Streptomyces griseoflavus
226
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme M199G, 30°C, pH not specified in the publication Streptomyces griseoflavus
261
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199G Streptomyces griseoflavus
278
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme Y230A Streptomyces griseoflavus
279
-
 K4[Fe(CN)6] 30°C, pH 4.0, mutant enzyme M199G/R240H Streptomyces griseoflavus
383
-
 K4[Fe(CN)6] 30°C, pH 4.0, wild-type enzyme Streptomyces griseoflavus