Application | Comment | Organism |
---|---|---|
analysis | rapid and simple determination of L-ascorbate in food with immobilized enzyme | Cucurbita moschata |
General Stability | Organism |
---|---|
immobilization within 6% Ca-alginate gel beads improves stability | Cucurbita moschata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita moschata | - |
- |
- |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
immobilized enzyme retains full activity for 3 months at pH 5.0-7.0, free enzyme looses 40-70% activity within one day at pH 5.0-7.0 | Cucurbita moschata |
60 | - |
free enzyme: 20 min, 90% loss of activity, immobilized enzyme: 10% loss of activity | Cucurbita moschata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Cucurbita moschata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | free enzyme: approx. 60% activity at pH 5.0, less than 10% activity at pH 9.0, immobilized enzyme: approx. 80% activity at pH 5.0, approx. 45% activity at pH 9.0 | Cucurbita moschata |