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Literature summary for 1.10.3.3 extracted from

  • Sereikaite, J.; Iljaseviciene, D.; Dienys, G.; Danilcenko, H.; Gavrilova, V.
    Ascorbate oxidase: Specificity and analytical application (1993), Appl. Biochem. Biotechnol., 43, 153-160.
No PubMed abstract available
Search for more literature for 1.10.3.3

Application

Application Comment Organism
analysis quantitative determination of ascorbic acid with enzyme elctrode containing enzyme immobilized on nylon membrane Cucurbita sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 chlorohydroquinone
-
 Cucurbita sp.
1.5
-
 L-ascorbic acid
-
 Cucurbita sp.

Metals/Ions

Metals/Ions Comment Organism Structure
copper
-
 Cucurbita sp.

Organism

Organism UniProt Comment Textmining
Cucurbita sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-dextran-silochrome Cucurbita sp.

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Cucurbita sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + O2
-
 Cucurbita sp. 2 L-dehydroascorbate + 2 H2O
-
 ?
chlorohydroquinone + O2 6% of activity with L-ascorbic acid Cucurbita sp. chloroquinone + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 no loss in activity after 30 min, approx. 55% activity after 30 min at 60°C, approx. 30% activity after 30 min at 65°C Cucurbita sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Cucurbita sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 rapid loss of activity below pH 4 and above pH 12 Cucurbita sp.