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Literature summary for 1.10.3.3 extracted from

  • Porto, T.; Marques, P.; Porto, C.; Moreira, K.; Lima-Filho, J.; Converti, A.; Pessoa, A.; Porto, A.
    Extraction of ascorbate oxidase from Cucurbita maxima by continuous process in perforated rotating disc contactor using aqueous two-phase systems (2009), Appl. Biochem. Biotechnol., 160, 1057-1064.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-ascorbic acid + O2 Cucurbita maxima
-
2 L-dehydroascorbic acid + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
liquid-liquid extraction in aqueous two-phase systems in continuous process using perforated rotating disc contactor with 10% (w/v) PEG 20000 and 25% (w/v) citrate Cucurbita maxima
pre-purification using a aqueous two-phase system (PEG/citrate) Cucurbita maxima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbic acid + O2
-
Cucurbita maxima 2 L-dehydroascorbic acid + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
ascorbate oxidase
-
Cucurbita maxima