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Literature summary for 1.10.3.3 extracted from

  • Braunschmid, V.; Fuerst, S.; Perz, V.; Zitzenbacher, S.; Hoyo, J.; Fernandez-Sanchez, C.; Tzanov, T.; Steinkellner, G.; Gruber, K.; Nyanhongo, G.S.; Ribitsch, D.; Guebitz, G.M.
    A fungal ascorbate oxidase with unexpected laccase activity (2020), Int. J. Mol. Sci., 21, 5754 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.3

Cloned(Commentary)

Cloned (Comment) Organism
best expressed in Pichia pastoris Aspergillus flavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 L-ascorbate pH 5.0, 30°C Aspergillus flavus
1.89
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.4, 30°C Aspergillus flavus

Metals/Ions

Metals/Ions Comment Organism Structure
copper multi-copper oxidase Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
-
-
Aspergillus flavus ATCC 200026
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus flavus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.56
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.4, 30°C Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Aspergillus flavus ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Aspergillus flavus ATCC 200026 ?
-
 ?
4 L-ascorbate + O2
-
 Aspergillus flavus 4 monodehydroascorbate + 2 H2O
-
 ?
4 L-ascorbate + O2
-
 Aspergillus flavus ATCC 200026 4 monodehydroascorbate + 2 H2O
-
 ?
additional information syringaldezine and guaiacol are not oxidized Aspergillus flavus ?
-
-
additional information syringaldezine and guaiacol are not oxidized Aspergillus flavus ATCC 200026 ?
-
-

Synonyms

Synonyms Comment Organism
Af_AO1
-
 Aspergillus flavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or L-ascorbate Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 24 h, the enzyme retains 70% of its ascorbate oxidase activity Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Aspergillus flavus
5
-
 substrate: L-ascorbate Aspergillus flavus