BRENDA - Enzyme Database show
show all sequences of 1.10.3.4

Isophenoxazine synthase apoenzyme from Pygnoporus coccineus

Nair, P.M.; Vining, I.C.; Biochim. Biophys. Acta 96, 318-327 (1965)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
apoenzyme
Trametes coccinea
Inhibitors
Inhibitors
Commentary
Organism
Structure
ascorbic acid
-
Trametes coccinea
CuSO4
-
Trametes coccinea
cysteine
-
Trametes coccinea
FeSO4
-
Trametes coccinea
glutathione
-
Trametes coccinea
N-ethylmaleimide
-
Trametes coccinea
Na3AsO3
-
Trametes coccinea
NaBH4
-
Trametes coccinea
o-aminophenol
substrate inhibition above 0.6 mM
Trametes coccinea
p-chloromercuribenzoate
-
Trametes coccinea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.264
-
FMN
-
Trametes coccinea
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
holoenzyme reconstituted by addition of riboflavin and Mn2+ required for maximal activity, ineffective in absence of FMN
Trametes coccinea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trametes coccinea
-
wood-rotting fungus
-
Purification (Commentary)
Commentary
Organism
partial
Trametes coccinea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Trametes coccinea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Trametes coccinea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 2-aminophenol + 2 O2
oxidation of o-aminophenol to o-quinoneimine and subsequent condensation of o-aminophenol to 2-amino-3H-isophenoxazin-3-one in absence of Mn2+ requires about 1 mol of riboflavin 5'-phosphate per mol of o-aminophenol oxidized, in presence of Mn2+ riboflavin 5'-phosphate acts as catalyst
396431
Trametes coccinea
isophenoxazine + 2 H2O + H2O2
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
FAD no action as cofactor
Trametes coccinea
riboflavin 5'-monophosphate
holoenzyme reconstituted by addition of riboflavin 5'-phosphate and Mn2+
Trametes coccinea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
FAD no action as cofactor
Trametes coccinea
riboflavin 5'-monophosphate
holoenzyme reconstituted by addition of riboflavin 5'-phosphate and Mn2+
Trametes coccinea
Crystallization (Commentary) (protein specific)
Crystallization
Organism
apoenzyme
Trametes coccinea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ascorbic acid
-
Trametes coccinea
CuSO4
-
Trametes coccinea
cysteine
-
Trametes coccinea
FeSO4
-
Trametes coccinea
glutathione
-
Trametes coccinea
N-ethylmaleimide
-
Trametes coccinea
Na3AsO3
-
Trametes coccinea
NaBH4
-
Trametes coccinea
o-aminophenol
substrate inhibition above 0.6 mM
Trametes coccinea
p-chloromercuribenzoate
-
Trametes coccinea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.264
-
FMN
-
Trametes coccinea
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
holoenzyme reconstituted by addition of riboflavin and Mn2+ required for maximal activity, ineffective in absence of FMN
Trametes coccinea
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Trametes coccinea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Trametes coccinea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Trametes coccinea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 2-aminophenol + 2 O2
oxidation of o-aminophenol to o-quinoneimine and subsequent condensation of o-aminophenol to 2-amino-3H-isophenoxazin-3-one in absence of Mn2+ requires about 1 mol of riboflavin 5'-phosphate per mol of o-aminophenol oxidized, in presence of Mn2+ riboflavin 5'-phosphate acts as catalyst
396431
Trametes coccinea
isophenoxazine + 2 H2O + H2O2
-
-
-
?
Other publictions for EC 1.10.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742653
Kumar
-
Structurally characterized mo ...
Streptomyces antibioticus
Inorg. Chim. Acta
464
49-54
2017
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1
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1
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743128
Maji
-
Synthesis, crystal structure, ...
Streptomyces antibioticus
J. Mol. Struct.
1146
821-827
2017
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1
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743665
Ghosh
-
Synthesis, characterization a ...
Streptomyces antibioticus
Polyhedron
123
162-175
2017
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1
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742261
Jia
Preparation and characterizat ...
Streptomyces antibioticus
Chem. Cent. J.
10
34
2016
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4
2
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1
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1
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2
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3
2
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2
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1
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2
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4
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2
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1
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1
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3
2
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2
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1
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2
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742320
Dey
-
Catechol oxidase and phenoxaz ...
Streptomyces antibioticus
Coord. Chem. Rev.
310
80-115
2016
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1
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743781
Mitra
-
Catecholase and phenoxazinone ...
Streptomyces antibioticus
RSC Adv.
6
58831-58838
2016
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1
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1
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1
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724838
Jia
-
Optimization of phenoxazinone ...
Streptomyces antibioticus
Electron. J. Biotechnol.
16
0000
2013
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1
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1
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1
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1
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1
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1
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1
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1
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713565
Le Roes-Hill
Phenoxazinone synthase: whats' ...
Bauhinia monandra, Streptomyces antibioticus, Streptomyces griseus subsp. griseus, Tecoma stans, Trametes coccinea
Trends Biotechnol.
27
248-258
2009
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49
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4
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20
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7
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20
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4
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4
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3
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3
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49
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4
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20
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20
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4
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4
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1
4
4
1
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674768
Suzuki
A novel o-aminophenol oxidase ...
Streptomyces griseus
J. Biol. Chem.
281
824-833
2006
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1
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7
6
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1
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4
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1
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2
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12
-
1
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1
6
1
1
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7
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1
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7
7
6
-
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1
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-
1
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-
2
-
12
-
1
-
1
6
1
1
-
-
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-
-
-
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-
396432
Rao
Studies on the metabolism of o ...
Bauhinia monandra
Arch. Biochem. Biophys.
118
388-394
1967
-
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-
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1
15
1
-
1
-
1
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1
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1
1
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1
1
2
-
1
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1
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1
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1
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1
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15
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1
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1
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1
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1
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1
1
2
-
1
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1
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-
-
396431
Nair
Isophenoxazine synthase apoenz ...
Trametes coccinea
Biochim. Biophys. Acta
96
318-327
1965
-
-
-
1
-
-
10
1
-
1
-
-
-
2
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
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-
2
-
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-
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-
2
1
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-
10
-
1
-
1
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-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
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-
-
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-
396430
Nair
Isophenoxazine synthase ...
Tecoma stans
Biochim. Biophys. Acta
81
507-516
1964
-
-
-
-
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7
-
-
1
-
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1
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1
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1
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1
1
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1
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1
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7
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1
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1
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1
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1
1
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