BRENDA - Enzyme Database show
show all sequences of 1.10.3.4

A novel o-aminophenol oxidase responsible for formation of the phenoxazinone chromophore of grixazone

Suzuki, H.; Furusho, Y.; Higashi, T.; Ohnishi, Y.; Horinouchi, S.; J. Biol. Chem. 281, 824-833 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)/pLysS cells
Streptomyces griseus
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-amino-4-hydroxybenzensulfonic acid
-
Streptomyces griseus
4-hydroxy-3-nitrobenzaldehyde
-
Streptomyces griseus
aniline
-
Streptomyces griseus
L-tyrosine
-
Streptomyces griseus
o-Nitrophenol
-
Streptomyces griseus
p-Hydroxybenzaldehyde
-
Streptomyces griseus
phenol
-
Streptomyces griseus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
3,4-Dihydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
0.58
-
3-amino-4-hydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
0.75
-
2-amino-4-methylphenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
3.5
-
o-aminophenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
5.5
-
L-Dopa
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
19
-
catechol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
Superdex 200 gel filtration
Streptomyces griseus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces griseus
-
-
-
Purification (Commentary)
Commentary
Organism
Cu2+-charged HiTrap chelating HP column chromatography, HiTrap Q column chromatography and Resource PHE column chromatography
Streptomyces griseus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0000035
-
crude cell extract, at 30C
Streptomyces griseus
0.066
-
after 19000fold purification, at 30C
Streptomyces griseus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-4-methylphenol + O2
-
674768
Streptomyces griseus
?
-
-
-
?
2-amino-4-methylphenol + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
3,4-dihydroxybenzaldehyde + O2
-
674768
Streptomyces griseus
?
-
-
-
?
3,4-dihydroxybenzaldehyde + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
3-amino-4-hydroxybenzaldehyde + O2
-
674768
Streptomyces griseus
?
-
-
-
?
catechol + O2
-
674768
Streptomyces griseus
?
-
-
-
?
catechol + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
L-DOPA + O2
-
674768
Streptomyces griseus
?
-
-
-
?
L-DOPA + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
o-aminophenol + O2
-
674768
Streptomyces griseus
2-aminophenoxazin-3-one + H2O
-
-
-
?
protocatechuic acid + O2
-
674768
Streptomyces griseus
?
-
-
-
?
protocatechuic acid + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
at pH 7.0
Streptomyces griseus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
the enzyme remains active below 55C at pH 7.0 for 20 min at various temperatures
Streptomyces griseus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.066
-
L-Dopa
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
0.8
-
3,4-Dihydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
12
-
catechol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
14
-
3-amino-4-hydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
18
-
2-amino-4-methylphenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
20
-
o-aminophenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10.5
-
Streptomyces griseus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11
-
Streptomyces griseus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
p-Hydroxybenzaldehyde
-
Streptomyces griseus
3.2
-
L-tyrosine
-
Streptomyces griseus
3.9
-
4-hydroxy-3-nitrobenzaldehyde
-
Streptomyces griseus
6.1
-
o-Nitrophenol
-
Streptomyces griseus
14
-
aniline
-
Streptomyces griseus
21
-
phenol
-
Streptomyces griseus
31
-
3-amino-4-hydroxybenzensulfonic acid
-
Streptomyces griseus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)/pLysS cells
Streptomyces griseus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-amino-4-hydroxybenzensulfonic acid
-
Streptomyces griseus
4-hydroxy-3-nitrobenzaldehyde
-
Streptomyces griseus
aniline
-
Streptomyces griseus
L-tyrosine
-
Streptomyces griseus
o-Nitrophenol
-
Streptomyces griseus
p-Hydroxybenzaldehyde
-
Streptomyces griseus
phenol
-
Streptomyces griseus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
p-Hydroxybenzaldehyde
-
Streptomyces griseus
3.2
-
L-tyrosine
-
Streptomyces griseus
3.9
-
4-hydroxy-3-nitrobenzaldehyde
-
Streptomyces griseus
6.1
-
o-Nitrophenol
-
Streptomyces griseus
14
-
aniline
-
Streptomyces griseus
21
-
phenol
-
Streptomyces griseus
31
-
3-amino-4-hydroxybenzensulfonic acid
-
Streptomyces griseus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
3,4-Dihydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
0.58
-
3-amino-4-hydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
0.75
-
2-amino-4-methylphenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
3.5
-
o-aminophenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
5.5
-
L-Dopa
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
19
-
catechol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
Superdex 200 gel filtration
Streptomyces griseus
Purification (Commentary) (protein specific)
Commentary
Organism
Cu2+-charged HiTrap chelating HP column chromatography, HiTrap Q column chromatography and Resource PHE column chromatography
Streptomyces griseus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0000035
-
crude cell extract, at 30C
Streptomyces griseus
0.066
-
after 19000fold purification, at 30C
Streptomyces griseus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-4-methylphenol + O2
-
674768
Streptomyces griseus
?
-
-
-
?
2-amino-4-methylphenol + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
3,4-dihydroxybenzaldehyde + O2
-
674768
Streptomyces griseus
?
-
-
-
?
3,4-dihydroxybenzaldehyde + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
3-amino-4-hydroxybenzaldehyde + O2
-
674768
Streptomyces griseus
?
-
-
-
?
catechol + O2
-
674768
Streptomyces griseus
?
-
-
-
?
catechol + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
L-DOPA + O2
-
674768
Streptomyces griseus
?
-
-
-
?
L-DOPA + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
o-aminophenol + O2
-
674768
Streptomyces griseus
2-aminophenoxazin-3-one + H2O
-
-
-
?
protocatechuic acid + O2
-
674768
Streptomyces griseus
?
-
-
-
?
protocatechuic acid + O2
-
674768
Streptomyces griseus IFO 13350
?
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
at pH 7.0
Streptomyces griseus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
the enzyme remains active below 55C at pH 7.0 for 20 min at various temperatures
Streptomyces griseus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.066
-
L-Dopa
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
0.8
-
3,4-Dihydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
12
-
catechol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
14
-
3-amino-4-hydroxybenzaldehyde
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
18
-
2-amino-4-methylphenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
20
-
o-aminophenol
in 50 mM sodium phosphate, pH 7.0, 30C
Streptomyces griseus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10.5
-
Streptomyces griseus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11
-
Streptomyces griseus
Other publictions for EC 1.10.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742653
Kumar
-
Structurally characterized mo ...
Streptomyces antibioticus
Inorg. Chim. Acta
464
49-54
2017
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
1
-
-
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743128
Maji
-
Synthesis, crystal structure, ...
Streptomyces antibioticus
J. Mol. Struct.
1146
821-827
2017
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
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-
1
-
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-
2
-
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-
-
-
-
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-
-
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-
743665
Ghosh
-
Synthesis, characterization a ...
Streptomyces antibioticus
Polyhedron
123
162-175
2017
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-
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-
1
-
1
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2
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1
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2
-
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-
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-
-
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-
-
-
742261
Jia
Preparation and characterizat ...
Streptomyces antibioticus
Chem. Cent. J.
10
34
2016
-
-
-
-
-
-
4
2
-
1
-
1
-
2
-
-
-
-
-
-
-
3
2
-
2
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
1
-
1
-
-
-
-
-
-
-
3
2
-
2
-
1
-
2
-
-
-
-
-
-
-
-
-
742320
Dey
-
Catechol oxidase and phenoxaz ...
Streptomyces antibioticus
Coord. Chem. Rev.
310
80-115
2016
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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-
-
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-
1
-
1
-
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-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
743781
Mitra
-
Catecholase and phenoxazinone ...
Streptomyces antibioticus
RSC Adv.
6
58831-58838
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
2
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1
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2
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-
-
-
-
-
-
-
724838
Jia
-
Optimization of phenoxazinone ...
Streptomyces antibioticus
Electron. J. Biotechnol.
16
0000
2013
-
-
1
-
-
-
-
-
-
-
-
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-
1
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-
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1
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1
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1
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1
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-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713565
Le Roes-Hill
Phenoxazinone synthase: whats' ...
Bauhinia monandra, Streptomyces antibioticus, Streptomyces griseus subsp. griseus, Tecoma stans, Trametes coccinea
Trends Biotechnol.
27
248-258
2009
-
-
-
-
-
-
49
-
-
4
-
20
-
7
-
-
-
-
-
-
-
-
20
-
4
-
-
-
4
-
-
3
-
-
-
-
-
-
3
-
-
-
-
49
-
-
-
4
-
20
-
-
-
-
-
-
-
-
20
-
4
-
-
-
4
-
-
-
1
4
4
1
-
-
674768
Suzuki
A novel o-aminophenol oxidase ...
Streptomyces griseus
J. Biol. Chem.
281
824-833
2006
-
-
1
-
-
-
7
6
-
-
1
-
-
4
-
-
1
-
-
-
2
-
12
-
1
-
1
6
1
1
-
-
7
-
-
-
-
1
-
-
-
-
-
7
7
6
-
-
1
-
-
-
-
1
-
-
2
-
12
-
1
-
1
6
1
1
-
-
-
-
-
-
-
-
396432
Rao
Studies on the metabolism of o ...
Bauhinia monandra
Arch. Biochem. Biophys.
118
388-394
1967
-
-
-
-
-
1
15
1
-
1
-
1
-
1
-
-
1
1
-
-
1
1
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
15
-
1
-
1
-
1
-
-
-
1
-
-
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
396431
Nair
Isophenoxazine synthase apoenz ...
Trametes coccinea
Biochim. Biophys. Acta
96
318-327
1965
-
-
-
1
-
-
10
1
-
1
-
-
-
2
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
10
-
1
-
1
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396430
Nair
Isophenoxazine synthase ...
Tecoma stans
Biochim. Biophys. Acta
81
507-516
1964
-
-
-
-
-
-
7
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-