BRENDA - Enzyme Database
show all sequences of 1.10.3.6

Rifamycin B oxidase from Monocillium spp., a new type of diphenol oxidase

Han, M.H.; Seong, B.L.; Son, H.J.; Mheen, T.I.; FEBS Lett. 151, 36-40 (1983)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
FAD
slight activation
Monocillium spp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
NADH
apparent inhibition
Monocillium spp.
NADPH
apparent inhibition
Monocillium spp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Monocillium spp.
reverse reaction of rifamycin B biosynthesis
rifamycin O + H2O2
-
Monocillium spp.
?
Organism
Organism
UniProt
Commentary
Textmining
Monocillium spp.
-
ATCC 20621
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, ion-exchange, gel filtration
Monocillium spp.
Reaction
Reaction
Commentary
Organism
Reaction ID
rifamycin B + O2 = rifamycin O + H2O2
acting on diphenols and related substances as electron donors and on oxygen as an electron acceptor
Monocillium spp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.19
-
-
Monocillium spp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
p-hydroquinone + O2
rapid oxidation
439882
Monocillium spp.
?
-
439882
Monocillium spp.
?
pyrogallol + ?
-
439882
Monocillium spp.
?
-
439882
Monocillium spp.
?
rifamycin B + O2
rapid oxidation
439882
Monocillium spp.
rifamycin O + H2O2
-
439882
Monocillium spp.
?
rifamycin B + O2
reverse reaction of rifamycin B biosynthesis
439882
Monocillium spp.
rifamycin O + H2O2
-
439882
Monocillium spp.
?
rifamycin SV + ?
-
439882
Monocillium spp.
?
-
439882
Monocillium spp.
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
40
assay at
Monocillium spp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Monocillium spp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
enzyme contains no flavins, heme, Cu2+ or other metal ions
Monocillium spp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
FAD
slight activation
Monocillium spp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
enzyme contains no flavins, heme, Cu2+ or other metal ions
Monocillium spp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADH
apparent inhibition
Monocillium spp.
NADPH
apparent inhibition
Monocillium spp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Monocillium spp.
reverse reaction of rifamycin B biosynthesis
rifamycin O + H2O2
-
Monocillium spp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, ion-exchange, gel filtration
Monocillium spp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.19
-
-
Monocillium spp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
p-hydroquinone + O2
rapid oxidation
439882
Monocillium spp.
?
-
439882
Monocillium spp.
?
pyrogallol + ?
-
439882
Monocillium spp.
?
-
439882
Monocillium spp.
?
rifamycin B + O2
rapid oxidation
439882
Monocillium spp.
rifamycin O + H2O2
-
439882
Monocillium spp.
?
rifamycin B + O2
reverse reaction of rifamycin B biosynthesis
439882
Monocillium spp.
rifamycin O + H2O2
-
439882
Monocillium spp.
?
rifamycin SV + ?
-
439882
Monocillium spp.
?
-
439882
Monocillium spp.
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
40
assay at
Monocillium spp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Monocillium spp.
Other publictions for EC 1.10.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688670
Jobanputra
-
Influence of nitrogen sources ...
Chryseobacterium sp.
J. Sci. Ind. Res.
66
615-617
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439887
Patil
-
Influence of substrate prepara ...
Curvularia lunata
Indian J. Exp. Biol.
35
917-919
1997
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439886
Banerjee
Transformation of rifamycin B ...
Curvularia lunata
Biotechnol. Tech.
7
339-344
1993
-
-
-
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
1
1
4
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
1
1
4
-
1
1
-
-
-
-
-
-
-
-
439888
Banerjee
Characterization of soluble ri ...
Curvularia lunata
Lett. Appl. Microbiol.
17
1-3
1993
-
1
-
-
-
-
5
1
1
1
-
1
-
7
-
-
1
-
-
-
-
-
1
-
-
1
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
1
1
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
439883
Vohra
-
Microbial transformation of ri ...
Curvularia lunata
Biotechnol. Lett.
11
851-854
1989
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
439884
Seong
-
Enzymatic oxidation of rifamyc ...
Humicola spp.
J. Ferment. Technol.
63
515-522
1985
-
1
-
-
-
-
3
1
1
1
-
1
-
1
-
-
1
1
-
-
-
1
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
1
1
1
-
1
-
-
-
1
-
-
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439885
Lee
-
The properties of immobilized ...
Humicola spp.
Biotechnol. Lett.
6
143-148
1984
-
1
-
-
-
-
1
2
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
2
-
1
-
1
1
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
2
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
1
-
1
1
-
-
-
-
-
-
-
-
439882
Han
Rifamycin B oxidase from Monoc ...
Monocillium spp.
FEBS Lett.
151
36-40
1983
1
-
-
-
-
-
2
-
-
-
-
1
-
4
-
-
1
1
-
-
1
-
5
-
-
1
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-
-
1
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1
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1
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-
1
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-
-
2
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1
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1
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1
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5
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1
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1
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