BRENDA - Enzyme Database
show all sequences of 1.10.3.6

Enzymatic oxidation of rifamycins by a microorganism of the genus Humicola

Seong, B.L.; Son, H.J.; Mheen, T.I.; Park, Y.H.; Han, M.H.; J. Ferment. Technol. 63, 515-522 (1985)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
rifamycin, an ansa-macrolide antibiotic, is used for production of antituberculosic agents
Humicola spp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Fe2+
-
Humicola spp.
Hg2+
-
Humicola spp.
Rifamycin B
concentration above 2 mM
Humicola spp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
Rifamycin B
-
Humicola spp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
-
Humicola spp.
5622
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
not affected by other metal ions than Fe2+, Hg2+
Humicola spp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Humicola spp.
-
rifamycin O + H2O2
-
Humicola spp.
?
Organism
Organism
UniProt
Commentary
Textmining
Humicola spp.
-
ATCC 20620
-
Purification (Commentary)
Purification (Commentary)
Organism
partially, ammonium sulfate precipitation
Humicola spp.
Reaction
Reaction
Commentary
Organism
Reaction ID
rifamycin B + O2 = rifamycin O + H2O2
acts on various kinds of diphenol compounds, converting them into quinonic forms and H2O2
Humicola spp.
Storage Stability
Storage Stability
Organism
4°C, 100 mM phosphate buffer, pH 7.8, one month, no appreciable loss of actvity
Humicola spp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
catechol + O2
-
439884
Humicola spp.
?
-
-
-
?
p-hydroquinone + O2
-
439884
Humicola spp.
?
-
439884
Humicola spp.
?
p-hydroxyphenoxyacetic acid + O2
-
439884
Humicola spp.
?
-
-
-
?
rifamycin B + O2
-
439884
Humicola spp.
rifamycin O + H2O2
-
439884
Humicola spp.
?
rifamycin B + O2
most specific, highest catalytic efficiency
439884
Humicola spp.
rifamycin O + H2O2
-
439884
Humicola spp.
?
rifamycin SV + ?
-
439884
Humicola spp.
?
-
439884
Humicola spp.
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
above the catalytic activity is greatly reduced
Humicola spp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8
-
Humicola spp.
Application (protein specific)
Application
Commentary
Organism
medicine
rifamycin, an ansa-macrolide antibiotic, is used for production of antituberculosic agents
Humicola spp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Fe2+
-
Humicola spp.
Hg2+
-
Humicola spp.
Rifamycin B
concentration above 2 mM
Humicola spp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
Rifamycin B
-
Humicola spp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
-
Humicola spp.
5622
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
not affected by other metal ions than Fe2+, Hg2+
Humicola spp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Humicola spp.
-
rifamycin O + H2O2
-
Humicola spp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially, ammonium sulfate precipitation
Humicola spp.
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 100 mM phosphate buffer, pH 7.8, one month, no appreciable loss of actvity
Humicola spp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
catechol + O2
-
439884
Humicola spp.
?
-
-
-
?
p-hydroquinone + O2
-
439884
Humicola spp.
?
-
439884
Humicola spp.
?
p-hydroxyphenoxyacetic acid + O2
-
439884
Humicola spp.
?
-
-
-
?
rifamycin B + O2
-
439884
Humicola spp.
rifamycin O + H2O2
-
439884
Humicola spp.
?
rifamycin B + O2
most specific, highest catalytic efficiency
439884
Humicola spp.
rifamycin O + H2O2
-
439884
Humicola spp.
?
rifamycin SV + ?
-
439884
Humicola spp.
?
-
439884
Humicola spp.
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
above the catalytic activity is greatly reduced
Humicola spp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8
-
Humicola spp.
Other publictions for EC 1.10.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688670
Jobanputra
-
Influence of nitrogen sources ...
Chryseobacterium sp.
J. Sci. Ind. Res.
66
615-617
2007
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1
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-
-
-
-
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439887
Patil
-
Influence of substrate prepara ...
Curvularia lunata
Indian J. Exp. Biol.
35
917-919
1997
-
1
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3
1
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3
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1
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1
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1
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439886
Banerjee
Transformation of rifamycin B ...
Curvularia lunata
Biotechnol. Tech.
7
339-344
1993
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1
1
4
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1
1
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439888
Banerjee
Characterization of soluble ri ...
Curvularia lunata
Lett. Appl. Microbiol.
17
1-3
1993
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1
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5
1
1
1
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1
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7
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1
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1
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1
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2
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1
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1
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5
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1
1
1
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1
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1
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1
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1
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2
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1
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439883
Vohra
-
Microbial transformation of ri ...
Curvularia lunata
Biotechnol. Lett.
11
851-854
1989
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1
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1
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1
1
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439884
Seong
-
Enzymatic oxidation of rifamyc ...
Humicola spp.
J. Ferment. Technol.
63
515-522
1985
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1
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3
1
1
1
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1
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6
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439885
Lee
-
The properties of immobilized ...
Humicola spp.
Biotechnol. Lett.
6
143-148
1984
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2
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439882
Han
Rifamycin B oxidase from Monoc ...
Monocillium spp.
FEBS Lett.
151
36-40
1983
1
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