BRENDA - Enzyme Database
show all sequences of 1.10.3.6

The properties of immobilized whole cell of Humicola spp. with rifamycin oxydase activity

Lee, G.M.; Choi, C.Y.; Biotechnol. Lett. 6, 143-148 (1984)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
rifamycin is used for the treatment of tuberculosis
Humicola spp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Rifamycin B
-
Humicola spp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
Rifamycin B
free acetone-defatted cells
Humicola spp.
0.6
-
Rifamycin B
immobilized acetone-defatted cells
Humicola spp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Humicola spp.
-
rifamycin O + H2O2
-
Humicola spp.
?
Organism
Organism
UniProt
Commentary
Textmining
Humicola spp.
-
ATCC 20620
-
Reaction
Reaction
Commentary
Organism
Reaction ID
rifamycin B + O2 = rifamycin O + H2O2
immobilized enzyme
Humicola spp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
rifamycin B + O2
-
439885
Humicola spp.
rifamycin O + H2O2
-
439885
Humicola spp.
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
assay at
Humicola spp.
50
-
-
Humicola spp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
pH 8.0, half life time of 8 days
Humicola spp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Humicola spp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
-
Humicola spp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
19.6
-
Rifamycin B
immobilized acetone-defatted cells
Humicola spp.
Application (protein specific)
Application
Commentary
Organism
medicine
rifamycin is used for the treatment of tuberculosis
Humicola spp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Rifamycin B
-
Humicola spp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
19.6
-
Rifamycin B
immobilized acetone-defatted cells
Humicola spp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
Rifamycin B
free acetone-defatted cells
Humicola spp.
0.6
-
Rifamycin B
immobilized acetone-defatted cells
Humicola spp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Humicola spp.
-
rifamycin O + H2O2
-
Humicola spp.
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
rifamycin B + O2
-
439885
Humicola spp.
rifamycin O + H2O2
-
439885
Humicola spp.
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
assay at
Humicola spp.
50
-
-
Humicola spp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
pH 8.0, half life time of 8 days
Humicola spp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Humicola spp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
-
Humicola spp.
Other publictions for EC 1.10.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688670
Jobanputra
-
Influence of nitrogen sources ...
Chryseobacterium sp.
J. Sci. Ind. Res.
66
615-617
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
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1
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-
-
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-
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-
-
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-
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-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439887
Patil
-
Influence of substrate prepara ...
Curvularia lunata
Indian J. Exp. Biol.
35
917-919
1997
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
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-
3
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439886
Banerjee
Transformation of rifamycin B ...
Curvularia lunata
Biotechnol. Tech.
7
339-344
1993
-
-
-
-
-
1
-
-
1
-
-
1
-
1
-
-
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1
-
-
-
-
2
-
-
1
1
4
-
1
1
-
-
-
-
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-
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-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
1
1
4
-
1
1
-
-
-
-
-
-
-
-
439888
Banerjee
Characterization of soluble ri ...
Curvularia lunata
Lett. Appl. Microbiol.
17
1-3
1993
-
1
-
-
-
-
5
1
1
1
-
1
-
7
-
-
1
-
-
-
-
-
1
-
-
1
-
2
-
1
-
-
-
-
-
-
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1
-
-
-
-
-
-
5
-
1
1
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
439883
Vohra
-
Microbial transformation of ri ...
Curvularia lunata
Biotechnol. Lett.
11
851-854
1989
-
1
-
-
-
-
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-
1
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1
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1
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-
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1
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1
1
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-
1
1
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1
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-
-
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-
1
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1
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1
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1
1
-
-
1
1
-
-
-
-
-
-
-
-
439884
Seong
-
Enzymatic oxidation of rifamyc ...
Humicola spp.
J. Ferment. Technol.
63
515-522
1985
-
1
-
-
-
-
3
1
1
1
-
1
-
1
-
-
1
1
-
-
-
1
6
-
-
1
-
-
-
1
-
-
-
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-
-
-
1
-
-
-
-
-
-
3
-
1
1
1
-
1
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-
1
-
-
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439885
Lee
-
The properties of immobilized ...
Humicola spp.
Biotechnol. Lett.
6
143-148
1984
-
1
-
-
-
-
1
2
-
-
-
1
-
1
-
-
-
1
-
-
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-
1
-
-
2
-
1
-
1
1
-
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1
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1
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-
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-
-
1
1
2
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-
1
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-
-
-
-
1
-
2
-
1
-
1
1
-
-
-
-
-
-
-
-
439882
Han
Rifamycin B oxidase from Monoc ...
Monocillium spp.
FEBS Lett.
151
36-40
1983
1
-
-
-
-
-
2
-
-
-
-
1
-
4
-
-
1
1
-
-
1
-
5
-
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1
-
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1
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1
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1
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1
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2
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1
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1
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1
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5
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1
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1
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