BRENDA - Enzyme Database
show all sequences of 1.10.3.6

Characterization of soluble rifamycin oxidase from Curvularia lunata var. aeria

Banerjee, U.C.; Lett. Appl. Microbiol. 17, 1-3 (1993)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
rifamycin S is used for the production of the antibiotic rifampicin for treatment of leprosy and tuberculosis
Curvularia lunata
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
60% inhibition at 20 mM
Curvularia lunata
Co2+
slight inhibition
Curvularia lunata
Cu2+
slight inhibition
Curvularia lunata
Fe2+
90% inhibition at 20 mM
Curvularia lunata
Hg2+
complete inhibition at 20 mM
Curvularia lunata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.67
0.81
Rifamycin B
-
Curvularia lunata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Curvularia lunata
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
not affected by other metal ions than Fe2+, Hg2+, Ag+, Cu2+, Co2+ or EDTA
Curvularia lunata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Curvularia lunata
-
rifamycin O + H2O2
-
Curvularia lunata
?
Organism
Organism
UniProt
Commentary
Textmining
Curvularia lunata
-
var. aeria
-
Purification (Commentary)
Purification (Commentary)
Organism
partially, gel filtration
Curvularia lunata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
rifamycin B + O2
-
439888
Curvularia lunata
rifamycin O + H2O2
-
439888
Curvularia lunata
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Curvularia lunata
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
half life of 9 days, after 7 days nearly 100% of activity is retained
Curvularia lunata
40
-
half life of 100 min, activity directly declined
Curvularia lunata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Curvularia lunata
Application (protein specific)
Application
Commentary
Organism
medicine
rifamycin S is used for the production of the antibiotic rifampicin for treatment of leprosy and tuberculosis
Curvularia lunata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
60% inhibition at 20 mM
Curvularia lunata
Co2+
slight inhibition
Curvularia lunata
Cu2+
slight inhibition
Curvularia lunata
Fe2+
90% inhibition at 20 mM
Curvularia lunata
Hg2+
complete inhibition at 20 mM
Curvularia lunata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.67
0.81
Rifamycin B
-
Curvularia lunata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Curvularia lunata
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
not affected by other metal ions than Fe2+, Hg2+, Ag+, Cu2+, Co2+ or EDTA
Curvularia lunata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifamycin B + O2
Curvularia lunata
-
rifamycin O + H2O2
-
Curvularia lunata
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially, gel filtration
Curvularia lunata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
rifamycin B + O2
-
439888
Curvularia lunata
rifamycin O + H2O2
-
439888
Curvularia lunata
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Curvularia lunata
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
half life of 9 days, after 7 days nearly 100% of activity is retained
Curvularia lunata
40
-
half life of 100 min, activity directly declined
Curvularia lunata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Curvularia lunata
Other publictions for EC 1.10.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688670
Jobanputra
-
Influence of nitrogen sources ...
Chryseobacterium sp.
J. Sci. Ind. Res.
66
615-617
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439887
Patil
-
Influence of substrate prepara ...
Curvularia lunata
Indian J. Exp. Biol.
35
917-919
1997
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439886
Banerjee
Transformation of rifamycin B ...
Curvularia lunata
Biotechnol. Tech.
7
339-344
1993
-
-
-
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
1
1
4
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
1
1
4
-
1
1
-
-
-
-
-
-
-
-
439888
Banerjee
Characterization of soluble ri ...
Curvularia lunata
Lett. Appl. Microbiol.
17
1-3
1993
-
1
-
-
-
-
5
1
1
1
-
1
-
7
-
-
1
-
-
-
-
-
1
-
-
1
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
1
1
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
439883
Vohra
-
Microbial transformation of ri ...
Curvularia lunata
Biotechnol. Lett.
11
851-854
1989
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
439884
Seong
-
Enzymatic oxidation of rifamyc ...
Humicola spp.
J. Ferment. Technol.
63
515-522
1985
-
1
-
-
-
-
3
1
1
1
-
1
-
1
-
-
1
1
-
-
-
1
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
1
1
1
-
1
-
-
-
1
-
-
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439885
Lee
-
The properties of immobilized ...
Humicola spp.
Biotechnol. Lett.
6
143-148
1984
-
1
-
-
-
-
1
2
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
2
-
1
-
1
1
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
2
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
1
-
1
1
-
-
-
-
-
-
-
-
439882
Han
Rifamycin B oxidase from Monoc ...
Monocillium spp.
FEBS Lett.
151
36-40
1983
1
-
-
-
-
-
2
-
-
-
-
1
-
4
-
-
1
1
-
-
1
-
5
-
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-