Literature summary for 1.11.1.1 extracted from

Crane, E.J.; Yeh, J.I.; Luba, J.; Claiborne, A.
Analysis of the kinetic and redox properties of the NADH peroxidase R303M mutant: correlation with the crystal structure (2000), Biochemistry, 39, 10353-10364.

Search for more literature for 1.11.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
-	Enterococcus faecalis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method	Enterococcus faecalis

Protein Variants

Protein Variants	Comment	Organism
H10Q	lower Km for H2O2	Enterococcus faecalis
R303M	significant conformational change, lower Km and kcat	Enterococcus faecalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00069	-	H2O2	H10Q mutant	Enterococcus faecalis
0.001	-	NADH	R303M mutant	Enterococcus faecalis
0.003	-	NADH	wild type	Enterococcus faecalis
0.0064	-	H2O2	R303M mutant	Enterococcus faecalis
0.0068	-	NADH	H10Q mutant	Enterococcus faecalis
0.014	-	H2O2	wild type	Enterococcus faecalis

Organism

Organism	UniProt	Comment	Textmining
Enterococcus faecalis	P37062	wild type and mutants	-

Purification (Commentary)

Purification (Comment)	Organism
-	Enterococcus faecalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + H2O2	-	Enterococcus faecalis	NAD+ + H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.05	-	NADH	R303M mutant	Enterococcus faecalis
0.388	-	NADH	wild type	Enterococcus faecalis
0.395	-	NADH	H10Q mutant	Enterococcus faecalis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Enterococcus faecalis

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Release 2023.1 (January 2023)