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Literature summary for 1.11.1.10 extracted from

  • Pesic, M.; Bozic, N.; Lopez, C.; Loncar, N.; Alvaro, G.; Vujcic, Z.
    Chemical modification of chloroperoxidase for enhanced stability and activity (2014), Process Biochem., 49, 1472-1479 .
No PubMed abstract available
Search for more literature for 1.11.1.10

Protein Variants

Protein Variants Comment Organism
additional information upon chemical modifications such as modifications of Lys residues with maleic and phthalic anhydride, of carboxyl groups with carbodiimide and ethanolamine or ethylenediamine, crosslinking with DMS and periodate oxidation, the loss of both chlorinating and peroxidative activity is observed for all types of modification, within the range of 39.5 and 56.8% for the chlorinating activity, and 16.4 and 44.5% for peroxidative activity Leptoxyphium fumago

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Leptoxyphium fumago
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl ethanolamine + tert-butyl hydroperoxide
-
 Leptoxyphium fumago benzyloxycarbonyl glycinal
-
 ?
guaiacol + H2O2
-
 Leptoxyphium fumago tetraguaiacol + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 5.0, native CPO, half-live 2 h. All chemically modified CPOs are more stable than the native CPO Leptoxyphium fumago

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 native CPO and chemically modified CPOs, almost completely stable at room temperature for 3 days Leptoxyphium fumago
7
-
 native CPO, half-live 5 h. For CPO modified by carbodiimide, ethanolamine and periodate oxidized CPO, half lives are 2.1- and 1.5fold improved Leptoxyphium fumago