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Literature summary for 1.11.1.10 extracted from

  • Zhu, X.; Fan, X.; Wang, Y.; Zhai, Q.; Hu, M.; Li, S.; Jiang, Y.
    Amino modified magnetic halloysite nanotube supporting chloroperoxidase immobilization enhanced stability, reusability, and efficient degradation of pesticide residue in wastewater (2021), Bioprocess Biosyst. Eng., 44, 483-493 .
    View publication on PubMed
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Application

Application Comment Organism
environmental protection amino modified magnetic halloysite nanotube supporting chloroperoxidase immobilization is useful for enhanced stability, reusability, and efficient degradation of pesticide residue in wastewater. Degradation of mesotrione in wastewater by the immobilized CPO Leptoxyphium fumago

Protein Variants

Protein Variants Comment Organism
additional information immobilization of chloroperoxidase, method development, detailed overview. Halloysite nanotube (HNT) is a natural bio-compatible and stable nanomaterial available in abundance at low-cost. HNT is modified by two strategies to make it suitable for supporting immobilization of chloroperoxidase (CPO). Firstly, Fe3O4- nanoparticles are deposited on HNT, so magnetic separation can be used instead of centrifugation. Secondly, the magnetic HNT is modified by 3-aminopropyltriethoxysilane (APTES), which can provide amine group on surface of HNT and meanwhile inhibit the agglomeration of magnetic HNT. Then, HNT-Fe3O4 -APTES is linked with branched polyethyleneimine (PEI) to provide more amino for binding with enzyme. The so-prepared resin (CPO-HNT-Fe3O4-APTES-PEI) shows enhanced enzyme loading, reusability, improved thermal stability, and tolerance to organic solvents than free CPO. The affinity and specificity of immobilized enzyme to substrate is increased, Michaelis-Menten kinetic analysis Leptoxyphium fumago

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetic analysis of free and immobilized enzymes, overview Leptoxyphium fumago

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leptoxyphium fumago the immobilized enzyme CPO catalyzes degradation of mesotrione (2-[(4-methylsulfonyl)-2-nitrobenzoyl]cyclohexan-1,3-dione) in wastewater ?
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-

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago P04963 Caldariomyces fumago
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the immobilized enzyme CPO catalyzes degradation of mesotrione (2-[(4-methylsulfonyl)-2-nitrobenzoyl]cyclohexan-1,3-dione) in wastewater Leptoxyphium fumago ?
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Synonyms

Synonyms Comment Organism
chloroperoxidase
-
 Leptoxyphium fumago
CPO
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 Leptoxyphium fumago

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 free CPO can remain only 16.2% of its initial activity after 1 h incubation at 70°C, while the immobilized enzyme can keep almost 100% of activity at the same condition Leptoxyphium fumago
90
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 free CPO can remain below 15% of its initial activity after 1 h incubation at 90°C, while the immobilized enzyme can keep about 30% of activity at the same condition Leptoxyphium fumago
100
-
 1 h, fee enzyme and immobilized enzyme, inactivation Leptoxyphium fumago

General Information

General Information Comment Organism
physiological function the immobilized enzyme CPO catalyzes degradation of mesotrione (2-[(4-methylsulfonyl)-2-nitrobenzoyl]cyclohexan-1,3-dione) in wastewater Leptoxyphium fumago