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Literature summary for 1.11.1.11 extracted from

  • Ohya, T.; Morimura, Y.; Saji, H.; Mihara, T.; Ikawa, T.
    Purification and characterization of ascorbate peroxidase in roots of Japanese radish (1997), Plant Sci., 125, 137-145.
No PubMed abstract available
Search for more literature for 1.11.1.11

General Stability

General Stability Organism
dialysis of crude enzyme preparations for 24 h against a buffer depleted of ascorbate or sorbitol results in 90% and 20% loss in enzyme activity, respectively. In air saturated solutions, salting-out results in a significant loss in enzyme activity, therefore exchange to N2 gas is performed prior to salting-out of the root enzyme Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol not inhibitory at 0.5 mM, 31% inhibition at 5 mM Raphanus sativus
5,5'-dithiobis(2-nitrobenzoic acid) 40% inhibition at 0.1 mM Raphanus sativus
cysteine 50% inhibition at 5 mM Raphanus sativus
dithioerythritol 67% inhibition at 0.05 mM Raphanus sativus
dithiothreitol 54% inhibition at 0.05 mM Raphanus sativus
EDTA not inhibitory Raphanus sativus
iodoacetate not inhibitory Raphanus sativus
KCN 95% inhibition at 0.1 mM Raphanus sativus
NaN3 17% inhibition at 1 mM, 87% inhibition at 10 mM Raphanus sativus
p-chloromercuribenzoate 87% inhibition at 0.005 mM, inactivation is partially reversible, 2-mercaptoethanol protects Raphanus sativus
reduced glutathione 33% inhibition at 5 mM Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 H2O2
-
 Raphanus sativus
0.77
-
 ascorbate
-
 Raphanus sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Raphanus sativus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
 Raphanus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 native PAGE Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate precipitation, dialysis and column chromatography on butyl-Toyopearl, DEAE-cellulofine and Sephadex G-75 Raphanus sativus

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
561.1
-
 purified enzyme Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytochrome c + H2O2 no activity Raphanus sativus ? + H2O
-
 ?
guaiacol + H2O2 no activity Raphanus sativus ?
-
 ?
L-ascorbate + H2O2 highly specific for L-ascorbate Raphanus sativus dehydroascorbate + H2O
-
 ?
additional information no activity with: cytochrome c, reduced glutathione, NADH, NADPH, 6-palmityl-ascorbate, ascorbate-2-sulfate, guaiacol, 3,3'-diaminobenzidine, pyrocatechol or D-iso-ascorbate Raphanus sativus ?
-
 ?
o-dianisidine + H2O2 the oxidation rate is only 8.6% of that with L-ascorbate Raphanus sativus ?
-
 ?
pyrogallol + H2O2 the oxidation rate is only 5.5% of that with L-ascorbate Raphanus sativus 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Raphanus sativus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Raphanus sativus