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Literature summary for 1.11.1.11 extracted from

  • Dai, Y.; Huang, C.; Wen, L.; Sheu, D.; Lin, C.
    Lemon ascorbate peroxidase: cDNA cloning and biochemical characterization (2012), Bot. Stud., 53, 9-17.
No PubMed abstract available
Search for more literature for 1.11.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Citrus limon

Crystallization (Commentary)

Crystallization (Comment) Organism
construction of a 3-D structural model based on the crystal structure of Pisum sativum Apx, PDB code 1APX Citrus limon

General Stability

General Stability Organism
enzyme retains 35% activity after chymotrypsin digestion at pH 8 and 37°C for 40 min Citrus limon

Inhibitors

Inhibitors Comment Organism Structure
imidazole enzyme shows a decrease in its activity with increasing imidazole concentration, approximately 50% activity is lost in the presence of 0.8 M imidazole Citrus limon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 H2O2 pH 7.4, 37°C Citrus limon
0.4
-
 L-ascorbate pH 7.4, 37°C Citrus limon

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28100
-
 ESI Q-TOF Citrus limon
29000
-
 1 * 29000, calculated, 1 * 29000, SDS-PAGE, His-tagged recombinant protein Citrus limon

Organism

Organism UniProt Comment Textmining
Citrus limon J7EIR8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
 Citrus limon 2 monodehydroascorbate + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, calculated, 1 * 29000, SDS-PAGE, His-tagged recombinant protein Citrus limon

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 half-life 6.5 min Citrus limon

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71.5
-
 L-ascorbate pH 7.4, 37°C Citrus limon
84.5
-
 H2O2 pH 7.4, 37°C Citrus limon

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Citrus limon

pI Value

Organism Comment pI Value Maximum pI Value
Citrus limon calculated
-
 5.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
178
-
 L-ascorbate pH 7.4, 37°C Citrus limon
768
-
 H2O2 pH 7.4, 37°C Citrus limon