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Literature summary for 1.11.1.11 extracted from

  • Denli, Z.; Dogru, M.; Arabaci, G.
    Extraction, characterization and inhibition sensitivity of ascorbate peroxidase from kiwano (Cucumis metuliferus) (2020), Fresenius Environ. Bull., 29, 1516-1521 .
No PubMed abstract available
Search for more literature for 1.11.1.11

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 70.3% activity remaining at 1 mM Cucumis metulifer
2-nitrobenzoic acid 1.48% activity remaining at 1 mM Cucumis metulifer
Al3+ inhibits the enzyme at 1-5 mM, almost complete inhibition at 10 mM Cucumis metulifer
Ca2+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Cd2+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Co2+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Cu2+ inhibits the enzyme at 1 mM, complete inhibition at 5 mM Cucumis metulifer
EDTA 85.1% activity remaining at 10 mM Cucumis metulifer
Fe3+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Hg2+ inhibits the enzyme strongly at 1-10 mM Cucumis metulifer
iodoacetamide 92.5% activity remaining at 1 mM Cucumis metulifer
K+ inhibits the enzyme at 1-10 mM Cucumis metulifer
KCN 13.3% activity remaining at 1 mM Cucumis metulifer
L-cysteine 51.8% activity remaining at 1 mM Cucumis metulifer
Li+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Mg2+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Mn2+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Na+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Ni2+ inhibits the enzyme at 1-10 mM Cucumis metulifer
Pb2+ inhibits the enzyme at 1-5 mM, almost complete inhibition at 10 mM Cucumis metulifer
Sn2+ inhibits the enzyme at 1 mM, complete inhibition at 5 mM Cucumis metulifer
Sodium azide 25.9% activity remaining at 1 mM Cucumis metulifer
Zn2+ inhibits the enzyme at 1-10 mM Cucumis metulifer

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Cucumis metulifer
0.065
-
 H2O2 pH 6.2, 30°C, cell-free enzyme extract Cucumis metulifer
0.285
-
 L-ascorbate pH 6.2, 30°C, cell-free enzyme extract Cucumis metulifer

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cucumis metulifer 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates the activity of APX from kiwano at 1-10 mM Cucumis metulifer
Fe2+ in the heme group, activates the activity of APX from kiwano at 1-10 mM Cucumis metulifer

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+ Cucumis metulifer
-
 L-ascorbate + L-dehydroascorbate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cucumis metulifer
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
 Cucumis metulifer L-ascorbate + L-dehydroascorbate + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
APX
-
 Cucumis metulifer
ascorbate peroxidase
-
 Cucumis metulifer

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Cucumis metulifer

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 over 50% of maximal activity within this range, cell-free enzyme extract, profile overview Cucumis metulifer

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Cucumis metulifer

pH Range

pH Minimum pH Maximum Comment Organism
4 8 over 20% of maximal activity within this range, cell-free enzyme extract, profile overview. Over 50% of maximal activity at pH 6.0-7.5 Cucumis metulifer

Cofactor

Cofactor Comment Organism Structure
heme
-
 Cucumis metulifer