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Literature summary for 1.11.1.11 extracted from
- Role of Musa paradisiaca ascorbate peroxidase in the transformation of methyl phenyl sulfide to its sulfoxide (2019), Int. J. Biol. Macromol., 122, 962-968 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state Michaelis-Menten kietics | Musa x paradisiaca | |
| 0.13 | - |
L-ascorbate | pH 7.0, 25°C | Musa x paradisiaca |  |
| 0.23 | - |
H2O2 | pH 7.0, 25°C | Musa x paradisiaca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | in the heme group | Musa x paradisiaca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 208900 | - |
native PAGE and mass spectrometry | Musa x paradisiaca |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbate + H2O2 + 2 H+ | Musa x paradisiaca | - |
L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Musa x paradisiaca | - |
the Musa paradisiaca plant is cut from the campus of IIT Delhi, India | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 152fold from leaf juice by ultrafiltration, dialysis, and anion exchange chromatography, to homogeneity | Musa x paradisiaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Musa x paradisiaca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000264 | - |
the specific activity of the purified native enzyme is very low, pH 7.0, 25°C | Musa x paradisiaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbate + H2O2 + 2 H+ | - |
Musa x paradisiaca | L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? | |
| 2 L-ascorbate + H2O2 + 2 H+ | the reaction is initiated by the addition of H2O2 and oxidation of ascorbate is monitored by measuring the decrease in absorbance at 290 nm | Musa x paradisiaca | L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? | |
| additional information | the enzyme transforms approximately 97% methyl phenyl sulfide to its sulfoxide. The product is a racemic mixture | Musa x paradisiaca | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 208900, SDS-PAGE | Musa x paradisiaca |
| More | amino acid sequence determination and analysis, the enzyme consists of 977 amino acid residues | Musa x paradisiaca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APX | - |
Musa x paradisiaca |
| ascorbate peroxidase | - |
Musa x paradisiaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Musa x paradisiaca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 17 | 37 | over 30% of maximal activity within this range, profile overview | Musa x paradisiaca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27.24 | - |
H2O2 | pH 7.0, 25°C | Musa x paradisiaca | |
| 40.42 | - |
L-ascorbate | pH 7.0, 25°C | Musa x paradisiaca | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Musa x paradisiaca |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | over 30% of maximal activity within this range, profile overview | Musa x paradisiaca |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Musa x paradisiaca | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | role of Musa paradisiaca ascorbate peroxidase in the transformation of methyl phenyl sulfide to its sulfoxide | Musa x paradisiaca |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 118.4 | - |
H2O2 | pH 7.0, 25°C | Musa x paradisiaca | |
| 310.9 | - |
L-ascorbate | pH 7.0, 25°C | Musa x paradisiaca | |
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