Cloned (Comment) | Organism |
---|---|
enzyme PtotAPX expression analysis in callus cultures, recombinant expression of GFP-tagged enzyme in tobacco plants, localization to the chloroplasts | Populus tomentosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression or suppression of PtoAPX in Populus tomentosa callus. Light-induced chloroplast development is enhanced in PtotAPX-overexpressing transgenic Populus tomentosa callus with lower levels of hydrogen peroxide, but is suppressed in PtotAPX antisense transgenic callus with higher levels of hydrogen peroxide. The suppression of light-induced chloroplast development in PtotAPX antisense transgenic callus is relieved by the exogenous reactive oxygen species scavenging agent N,N'-dimethylthiourea (DMTU) | Populus tomentosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Populus tomentosa | 9507 | - |
additional information | transcriptome analysis during light-induced chloroplast development, detailed overview | Populus tomentosa | - |
- |
thylakoid | - |
Populus tomentosa | 9579 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in the heme group | Populus tomentosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | Populus tomentosa | - |
L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tomentosa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Populus tomentosa | - |
leaf | - |
Populus tomentosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | - |
Populus tomentosa | L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PtotAPX | - |
Populus tomentosa |
thylakoid ascorbate peroxidase | - |
Populus tomentosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Populus tomentosa |
General Information | Comment | Organism |
---|---|---|
malfunction | light-induced chloroplast development is enhanced in PtotAPX-overexpressing transgenic Populus tomentosa callus with lower levels of hydrogen peroxide, but is suppressed in PtotAPX antisense transgenic callus with higher levels of hydrogen peroxide | Populus tomentosa |
metabolism | chloroplasts with elaborate thylakoids can develop from proplastids in the cells of calli derived from leaf tissues of Populus tomentosa upon exposure to light. Chloroplast development is confirmed at the molecular and cellular levels and transcriptome analysis reveals that genes related to photoreceptors and photosynthesis are significantly upregulated during chloroplast development in a time-dependent manner. In light-induced chloroplast development, a key process is the removal of hydrogen peroxide, in which thylakoid-localized PtotAPX plays a major role | Populus tomentosa |
physiological function | chloroplast development is a complex process that is critical to the growth and development of plants, overview. Chloroplast thylakoid ascorbate peroxidase PtotAPX plays a key role in chloroplast development from proplastids upon exposure to light in Populus tomentosa and in thylakoid development by decreasing hydrogen peroxide | Populus tomentosa |