Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Sus scrofa | 5829 | - |
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Sus scrofa | - |
- |
microsome | - |
Bos taurus | - |
- |
microsome | - |
Canis lupus familiaris | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Se | enzyme contains a selenocysteine residue at the active site | Rattus norvegicus | |
Se | enzyme contains a selenocysteine residue at the active site | Sus scrofa | |
Se | enzyme contains a selenocysteine residue at the active site | Bos taurus | |
Se | enzyme contains a selenocysteine residue at the active site | Canis lupus familiaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
- |
Rattus norvegicus |
20000 | - |
- |
Sus scrofa |
20000 | - |
- |
Bos taurus |
20000 | - |
- |
Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + lipid hydroperoxide | Rattus norvegicus | protection of biomembranes against oxidative damage | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | Sus scrofa | protection of biomembranes against oxidative damage | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | Bos taurus | protection of biomembranes against oxidative damage | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | Canis lupus familiaris | protection of biomembranes against oxidative damage | glutathione disulfide + lipid + H2O | - |
? | |
additional information | Rattus norvegicus | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | ? | - |
? | |
additional information | Sus scrofa | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | ? | - |
? | |
additional information | Bos taurus | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | ? | - |
? | |
additional information | Canis lupus familiaris | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Canis lupus familiaris | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 glutathione + a hydroperoxy-fatty-acyl-[lipid] = glutathione disulfide + a hydroxy-fatty-acyl-[lipid] + H2O | ping-pong mechanism with formation of ternary complexes | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Sus scrofa | - |
brain | - |
Bos taurus | - |
brain | - |
Canis lupus familiaris | - |
heart | - |
Rattus norvegicus | - |
heart | - |
Sus scrofa | - |
heart | - |
Bos taurus | - |
kidney | - |
Rattus norvegicus | - |
kidney | - |
Sus scrofa | - |
kidney | - |
Bos taurus | - |
kidney | - |
Canis lupus familiaris | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
liver | - |
Bos taurus | - |
liver | - |
Canis lupus familiaris | - |
lung | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
spermatozoon | - |
Rattus norvegicus | - |
spermatozoon | - |
Bos taurus | - |
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0062 | - |
- |
Rattus norvegicus |
310.1 | - |
purified enzyme | Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 10% glycerol, stable for several months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + cholesterol hydroperoxides | - |
Rattus norvegicus | ? | - |
? | |
glutathione + cholesterol hydroperoxides | - |
Sus scrofa | ? | - |
? | |
glutathione + cholesterol hydroperoxides | - |
Bos taurus | ? | - |
? | |
glutathione + cholesterol hydroperoxides | - |
Canis lupus familiaris | ? | - |
? | |
glutathione + fatty acid hydroperoxides | - |
Sus scrofa | ? | - |
? | |
glutathione + H2O2 | - |
Rattus norvegicus | ? | - |
? | |
glutathione + H2O2 | - |
Sus scrofa | ? | - |
? | |
glutathione + H2O2 | - |
Bos taurus | ? | - |
? | |
glutathione + H2O2 | - |
Canis lupus familiaris | ? | - |
? | |
glutathione + lipid hydroperoxide | - |
Rattus norvegicus | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | - |
Bos taurus | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | - |
Canis lupus familiaris | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | wide specificity for lipid hydroperoxides | Sus scrofa | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | the oxidized active site of the enzyme can also be reduced by mercaptoethanol | Sus scrofa | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | protection of biomembranes against oxidative damage | Rattus norvegicus | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | protection of biomembranes against oxidative damage | Sus scrofa | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | protection of biomembranes against oxidative damage | Bos taurus | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | protection of biomembranes against oxidative damage | Canis lupus familiaris | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + phospholipid hydroperoxides | - |
Rattus norvegicus | ? | - |
? | |
glutathione + phospholipid hydroperoxides | - |
Sus scrofa | ? | - |
? | |
glutathione + phospholipid hydroperoxides | - |
Bos taurus | ? | - |
? | |
glutathione + tert-butyl hydroperoxide | - |
Rattus norvegicus | ? | - |
? | |
glutathione + tert-butyl hydroperoxide | - |
Sus scrofa | ? | - |
? | |
glutathione + tert-butyl hydroperoxide | - |
Bos taurus | ? | - |
? | |
glutathione + tert-butyl hydroperoxide | - |
Canis lupus familiaris | ? | - |
? | |
additional information | reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives | Rattus norvegicus | ? | - |
? | |
additional information | reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives | Sus scrofa | ? | - |
? | |
additional information | reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives | Bos taurus | ? | - |
? | |
additional information | reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives | Canis lupus familiaris | ? | - |
? | |
additional information | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | Rattus norvegicus | ? | - |
? | |
additional information | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | Sus scrofa | ? | - |
? | |
additional information | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | Bos taurus | ? | - |
? | |
additional information | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | Canis lupus familiaris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Rattus norvegicus |
monomer | - |
Bos taurus |
monomer | - |
Canis lupus familiaris |
monomer | 1 * 20000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
7.4 | - |
assay at | Sus scrofa |
7.4 | - |
assay at | Bos taurus |
7.4 | - |
assay at | Canis lupus familiaris |