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Literature summary for 1.11.1.12 extracted from

  • Maiorino, M.; Gregolin, C.; Ursini, F.
    Phospholipid hydroperoxide glutathione peroxidase (1990), Methods Enzymol., 186, 448-457.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-
cytosol
-
Sus scrofa 5829
-
microsome
-
Rattus norvegicus
-
-
microsome
-
Sus scrofa
-
-
microsome
-
Bos taurus
-
-
microsome
-
Canis lupus familiaris
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Se enzyme contains a selenocysteine residue at the active site Rattus norvegicus
Se enzyme contains a selenocysteine residue at the active site Sus scrofa
Se enzyme contains a selenocysteine residue at the active site Bos taurus
Se enzyme contains a selenocysteine residue at the active site Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
-
Rattus norvegicus
20000
-
-
Sus scrofa
20000
-
-
Bos taurus
20000
-
-
Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + lipid hydroperoxide Rattus norvegicus protection of biomembranes against oxidative damage glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide Sus scrofa protection of biomembranes against oxidative damage glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide Bos taurus protection of biomembranes against oxidative damage glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide Canis lupus familiaris protection of biomembranes against oxidative damage glutathione disulfide + lipid + H2O
-
?
additional information Rattus norvegicus enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes ?
-
?
additional information Sus scrofa enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes ?
-
?
additional information Bos taurus enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes ?
-
?
additional information Canis lupus familiaris enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Canis lupus familiaris
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
2 glutathione + a hydroperoxy-fatty-acyl-[lipid] = glutathione disulfide + a hydroxy-fatty-acyl-[lipid] + H2O ping-pong mechanism with formation of ternary complexes Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
brain
-
Sus scrofa
-
brain
-
Bos taurus
-
brain
-
Canis lupus familiaris
-
heart
-
Rattus norvegicus
-
heart
-
Sus scrofa
-
heart
-
Bos taurus
-
kidney
-
Rattus norvegicus
-
kidney
-
Sus scrofa
-
kidney
-
Bos taurus
-
kidney
-
Canis lupus familiaris
-
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-
liver
-
Bos taurus
-
liver
-
Canis lupus familiaris
-
lung
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-
spermatozoon
-
Rattus norvegicus
-
spermatozoon
-
Bos taurus
-
testis
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0062
-
-
Rattus norvegicus
310.1
-
purified enzyme Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 10% glycerol, stable for several months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + cholesterol hydroperoxides
-
Rattus norvegicus ?
-
?
glutathione + cholesterol hydroperoxides
-
Sus scrofa ?
-
?
glutathione + cholesterol hydroperoxides
-
Bos taurus ?
-
?
glutathione + cholesterol hydroperoxides
-
Canis lupus familiaris ?
-
?
glutathione + fatty acid hydroperoxides
-
Sus scrofa ?
-
?
glutathione + H2O2
-
Rattus norvegicus ?
-
?
glutathione + H2O2
-
Sus scrofa ?
-
?
glutathione + H2O2
-
Bos taurus ?
-
?
glutathione + H2O2
-
Canis lupus familiaris ?
-
?
glutathione + lipid hydroperoxide
-
Rattus norvegicus glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide
-
Bos taurus glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide
-
Canis lupus familiaris glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide wide specificity for lipid hydroperoxides Sus scrofa glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide the oxidized active site of the enzyme can also be reduced by mercaptoethanol Sus scrofa glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide protection of biomembranes against oxidative damage Rattus norvegicus glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide protection of biomembranes against oxidative damage Sus scrofa glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide protection of biomembranes against oxidative damage Bos taurus glutathione disulfide + lipid + H2O
-
?
glutathione + lipid hydroperoxide protection of biomembranes against oxidative damage Canis lupus familiaris glutathione disulfide + lipid + H2O
-
?
glutathione + phospholipid hydroperoxides
-
Rattus norvegicus ?
-
?
glutathione + phospholipid hydroperoxides
-
Sus scrofa ?
-
?
glutathione + phospholipid hydroperoxides
-
Bos taurus ?
-
?
glutathione + tert-butyl hydroperoxide
-
Rattus norvegicus ?
-
?
glutathione + tert-butyl hydroperoxide
-
Sus scrofa ?
-
?
glutathione + tert-butyl hydroperoxide
-
Bos taurus ?
-
?
glutathione + tert-butyl hydroperoxide
-
Canis lupus familiaris ?
-
?
additional information reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives Rattus norvegicus ?
-
?
additional information reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives Sus scrofa ?
-
?
additional information reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives Bos taurus ?
-
?
additional information reduces hydroxyperoxide derivatives of phospholipids into alcohol derivatives Canis lupus familiaris ?
-
?
additional information enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes Rattus norvegicus ?
-
?
additional information enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes Sus scrofa ?
-
?
additional information enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes Bos taurus ?
-
?
additional information enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes Canis lupus familiaris ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Rattus norvegicus
monomer
-
Bos taurus
monomer
-
Canis lupus familiaris
monomer 1 * 20000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus
7.4
-
assay at Sus scrofa
7.4
-
assay at Bos taurus
7.4
-
assay at Canis lupus familiaris