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Literature summary for 1.11.1.12 extracted from
- Purification of a cytosolic enzyme from human liver with phospholipid hydroperoxide glutathione peroxidase activity (1994), Int. J. Biochem., 26, 1279-1286.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholate | in reaction with cumene hydroperoxide and linoleic acid hydroperoxide | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Se | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16100 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + lipid hydroperoxide | Homo sapiens | protection of biomembranes against oxidative damage | glutathione disulfide + lipid + H2O | - |
? | |
| additional information | Homo sapiens | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | ? | - |
? | |
| additional information | Homo sapiens | enzyme inhibits peroxidation of microsomes | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.009 | - |
- |
Homo sapiens |
| 160 | - |
purified enzyme, substrate linoleic acid hydroperoxide | Homo sapiens |
| 190 | - |
purified enzyme, substrate cumene hydroperoxide | Homo sapiens |
| 210 | - |
purified enzyme, substrate H2O2 | Homo sapiens |
| 336 | - |
purified enzyme, substrate (beta-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatidylcholine | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -40°C, about 30% loss of activity during 1 freeze-thaw-cycle | Homo sapiens |
| 4°C, loss of 10% activity in 1 week | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + (beta-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatidylcholine | - |
Homo sapiens | ? | - |
? | |
| glutathione + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-3-phosphatidylcholine | - |
Homo sapiens | ? | - |
? | |
| glutathione + cumene hydroperoxide | - |
Homo sapiens | ? | - |
? | |
| glutathione + H2O2 | - |
Homo sapiens | ? | - |
? | |
| glutathione + linoleic acid hydroperoxide | - |
Homo sapiens | ? | - |
? | |
| glutathione + lipid hydroperoxide | - |
Homo sapiens | glutathione disulfide + lipid + H2O | - |
? | |
| glutathione + lipid hydroperoxide | protection of biomembranes against oxidative damage | Homo sapiens | glutathione disulfide + lipid + H2O | - |
? | |
| additional information | enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes | Homo sapiens | ? | - |
? | |
| additional information | enzyme inhibits peroxidation of microsomes | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 91 | - |
(beta-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatidylcholine | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Homo sapiens |
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