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Literature summary for 1.11.1.12 extracted from
- Enzymatic properties of a recombinant phospholipid hydroperoxide glutathione peroxidase from Momordica charantia and its complementation function in yeast (2009), Biochemistry (Moscow), 74, 502-508.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells, into the vector YEpGAP112 for a functional complementation test in yeast | Momordica charantia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01113 | - |
phosphatidylcholine hydroperoxide | apparent KM | Momordica charantia |  |
| 0.6383 | - |
H2O2 | apparent KM | Momordica charantia | |
| 2.932 | - |
tert-butyl-hydroperoxide | apparent KM | Momordica charantia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| selenium | - |
Momordica charantia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
His6-McPHGPx fusion protein, determined by SDS-PAGE | Momordica charantia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + a lipid hydroperoxide | Momordica charantia | - |
glutathione disulfide + lipid + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Momordica charantia | Q8W259 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by nickel affinity chromatography | Momordica charantia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + a lipid hydroperoxide | - |
Momordica charantia | glutathione disulfide + lipid + H2O | - |
? | |
| glutathione + H2O2 | - |
Momordica charantia | ? | - |
? | |
| glutathione + phosphatidylcholine hydroperoxide | - |
Momordica charantia | ? | - |
? | |
| glutathione + tert-butyl-hydroperoxide | - |
Momordica charantia | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Momordica charantia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| McPHGPx | - |
Momordica charantia |
| phospholipid hydroperoxide glutathione peroxidase | - |
Momordica charantia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
activity assay | Momordica charantia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | - |
Momordica charantia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
activity assay | Momordica charantia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.5 | - |
Momordica charantia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | - |
Momordica charantia | |
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Release 2023.1 (January 2023)
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