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Literature summary for 1.11.1.12 extracted from

  • Kernstock, R.M.; Girotti, A.W.
    New strategies for the isolation and activity determination of naturally occurring type-4 glutathione peroxidase (2008), Protein Expr. Purif., 62, 216-222.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Sephadex G-50 gel filtration, and HiTrap SP column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.051
-
 enzyme from tissue lysate, using L-alpha-phosphatidylcholine hydroperoxide as peroxide substrate, at 37°C Rattus norvegicus
21.5
-
 enzyme after 422fold purification, using L-alpha-phosphatidylcholine hydroperoxide as peroxide substrate, at 37°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 3beta-hydroxycholest-5-ene-7alpha-hydroperoxide
-
 Rattus norvegicus cholest-5-ene-3beta,7alpha-diol + ?
-
 ?
glutathione + cumene hydroperoxide
-
 Rattus norvegicus ?
-
 ?
glutathione + L-alpha-phosphatidylcholine hydroperoxide
-
 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
GPx4
-
 Rattus norvegicus
phospholipid hydroperoxide glutathione peroxidase
-
 Rattus norvegicus
type-4 glutathione peroxidase
-
 Rattus norvegicus