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Literature summary for 1.11.1.12 extracted from
- Crystal structure and functional characterization of selenocysteine-containing glutathione peroxidase 4 suggests an alternative mechanism of peroxide reduction (2018), Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1863, 1095-1107 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in a cysteine-auxotrophic Escherichia coli strain | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure of recombinant protein, at 1.3 A resolution. Data indicate a monomeric protein, which contains the catalytic selenium at the redox level of the seleninic acid. The presumed catalytic triad consists of residues Sec(Cys)46, Gln81 and Trp136. The triad is localized at a flat impression of the protein surface | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q81A | no residual activity | Homo sapiens |
| Q81E | no residual activity | Homo sapiens |
| Q81L | no residual activity | Homo sapiens |
| U46A | no residual activity | Homo sapiens |
| U46C | less than 1 % of residual activity | Homo sapiens |
| U46S | no residual activity | Homo sapiens |
| W136A | almost complete loss of activity | Homo sapiens |
| W136F | almost complete loss of activity | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| selenium | about 50% selenium incorporation, recombinant protein | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P36969 | isoform Gpx4 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14 | - |
substrate tert-butyl hydroperoxide, pH 7.4, 37°C | Homo sapiens |
| 25 | - |
substrate phosphatidylcholine hydroperoxide, pH 7.4, 37°C | Homo sapiens |
| 56 | - |
substrate H2O2, pH 7.4, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 glutathione + H2O2 | - |
Homo sapiens | glutathione disulfide + 2 H2O | - |
? | |
| 2 glutathione + phosphatidylcholine hydroperoxide | - |
Homo sapiens | glutathione disulfide + ? + H2O | - |
? | |
| 2 glutathione + tert-butyl hydroperoxide | - |
Homo sapiens | glutathione disulfide + 2-methylpropan-2-ol + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, SDS-PAGE, recombinant protein | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GPx4 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
less than 10% of maximum activity | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.1 | - |
tert-butyl hydroperoxide | pH 7.4, 37°C | Homo sapiens | |
| 9.2 | - |
phosphatidylcholine hydroperoxide | pH 7.4, 37°C | Homo sapiens | |
| 20.6 | - |
H2O2 | pH 7.4, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Homo sapiens |
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