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Literature summary for 1.11.1.13 extracted from
- Mn-dependent peroxidase from the lignin-degrading white rot fungus Phlebia radiata (1990), Arch. Biochem. Biophys., 279, 25-31.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | H2O2-dependent | Phlebia radiata |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| catalase | inhibits oxidation of vanillylacetone completely, oxidation of NADH at high concentrations partially | Phlebia radiata | |
| diphosphate | inhibits phenol red oxidation to 50%, NADH oxidation at high concentrations partially | Phlebia radiata | |
| additional information | not inhibited by sodium diphosphate, mannitol, Mn3+-chelators | Phlebia radiata | |
| Superoxide dismutase | inhibits phenol red oxidation to 50%, vanillylacetone oxidation to 90%, NADH oxidation at high concentrations partially | Phlebia radiata |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Phlebia radiata | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
x * 49000, SDS-PAGE | Phlebia radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phlebia radiata | - |
white rot fungus, strain 79 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 10% w/w carbohydrates | Phlebia radiata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phlebia radiata |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O | mechanism | Phlebia radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
| Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes phenol red | ? | |
| Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
| Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes several methoxylated and hydroxylated phenolic compounds | ? | |
| additional information | poor substrates: benzoate, benzaldehyde or benzyl alcohol | Phlebia radiata | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phlebia radiata | ? | - |
? | |
| additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates | Phlebia radiata | ? | - |
? | |
| additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phlebia radiata | ? | - |
? | |
| additional information | no activity with veratryl alcohol | Phlebia radiata | ? | - |
? | |
| additional information | Mn-dependent oxidation of phenols requires superoxide anion and H2O2, phenolic hydroxyl group is essential | Phlebia radiata | ? | - |
? | |
| additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phlebia radiata | ? | - |
? | |
| additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phlebia radiata | ? | - |
? | |
| additional information | poor substrates: benzoate, benzaldehyde or benzyl alcohol | Phlebia radiata 79 / ATCC 64658 | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phlebia radiata 79 / ATCC 64658 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, SDS-PAGE | Phlebia radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Phlebia radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 3.8 | Phlebia radiata |
| 4 | - |
oxidation of NADH | Phlebia radiata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | stable | Phlebia radiata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | iron protoporphyrin IX prosthetic group | Phlebia radiata | |
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