Activating Compound | Comment | Organism | Structure |
---|---|---|---|
malonate | - |
Phanerodontia chrysosporium | |
oxalate | activates by chelating and stabilizing Mn3+ | Phanerodontia chrysosporium |
Cloned (Comment) | Organism |
---|---|
cDNA sequences of several MnP-encoding genes, including mnp1, mnp2 and mnp3 | Phanerodontia chrysosporium |
Crystallization (Comment) | Organism |
---|---|
- |
Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
E35Q | engineered mutant | Phanerodontia chrysosporium |
R177A | mutant with reduced binding efficiency for Mn2+: disruption in the salt-bridge between Arg-177 and the Mn2+ binding ligand Glu-35 | Phanerodontia chrysosporium |
R177D | mutant with decreased electron-transfer rate and reduced binding efficiency for Mn2+: disruption in the salt-bridge between Arg-177 and the Mn2+ binding ligand Glu-35, higher redox potential for the enzyme-bound Mn2+ | Phanerodontia chrysosporium |
R177E | mutant with decreased electron-transfer rate and reduced binding efficiency for Mn2+: disruption in the salt-bridge between Arg-177 and the Mn2+ binding ligand Glu-35, higher redox potential for the enzyme-bound Mn2+ | Phanerodontia chrysosporium |
R177K | mutant with reduced binding efficiency for Mn2+: disruption in the salt-bridge between Arg-177 and the Mn2+ binding ligand Glu-35 | Phanerodontia chrysosporium |
R177N | mutant with decreased electron-transfer rate and reduced binding efficiency for Mn2+: disruption in the salt-bridge between Arg-177 and the Mn2+ binding ligand Glu-35, higher redox potential for the enzyme-bound Mn2+ | Phanerodontia chrysosporium |
R177Q | mutant with decreased electron-transfer rate and reduced binding efficiency for Mn2+: disruption in the salt-bridge between Arg-177 and the Mn2+ binding ligand Glu-35, higher redox potential for the enzyme-bound Mn2+ | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
H2O2 | mutant R177E | Phanerodontia chrysosporium | |
0.0078 | - |
H2O2 | mutant R177Q | Phanerodontia chrysosporium | |
0.0121 | - |
H2O2 | mutant R177N | Phanerodontia chrysosporium | |
0.0126 | - |
H2O2 | mutant R177D | Phanerodontia chrysosporium | |
0.039 | - |
H2O2 | mutant R177A | Phanerodontia chrysosporium | |
0.044 | - |
H2O2 | mutant R177K | Phanerodontia chrysosporium | |
0.055 | - |
H2O2 | wild-type MnP | Phanerodontia chrysosporium | |
0.09 | - |
Mn2+ | wild-type MnP | Phanerodontia chrysosporium | |
1.64 | - |
Mn2+ | mutant R177A | Phanerodontia chrysosporium | |
2.15 | - |
Mn2+ | mutant R177D | Phanerodontia chrysosporium | |
2.32 | - |
Mn2+ | mutant R177K | Phanerodontia chrysosporium | |
2.91 | - |
Mn2+ | mutant R177Q | Phanerodontia chrysosporium | |
3.61 | - |
Mn2+ | mutant R177N | Phanerodontia chrysosporium | |
3.9 | - |
Mn2+ | mutant R177E | Phanerodontia chrysosporium | |
4.4 | - |
Mn2+ | mutant E35Q | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, wild-type and mutants R177D, R177E, R177N and R177Q, SDS-PAGE | Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | important component of lignin degradation system | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Phanerodontia chrysosporium | - |
white rot basidomycete | - |
Phanerodontia chrysosporium | - |
MnP1 | - |
Phanerodontia chrysosporium | - |
auxotrophic strain OGC107-1 | - |
Phanerodontia chrysosporium OGC101 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the mutant enzymes R177D, R177E, R177N and R177Q | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium | Mn3+ + H2O | freely diffusible, enzyme-generated Mn(III)-organic-acid complex oxidizes phenolic substrates | ? | |
Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes lignin | ? | |
Mn2+ + H+ + H2O2 | role for Arg-177 in promoting efficient Mn2+ binding and oxidation by MnP | Phanerodontia chrysosporium | Mn3+ + H2O | freely diffusible, enzyme-generated Mn(III)-organic-acid complex oxidizes phenolic substrates | ? | |
Mn2+ + H+ + H2O2 | role for Arg-177 in promoting efficient Mn2+ binding and oxidation by MnP | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes lignin | ? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | freely diffusible, enzyme-generated Mn(III)-organic-acid complex oxidizes phenolic substrates | ? | |
additional information | structural properties | Phanerodontia chrysosporium | ? | - |
? | |
additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes ferrocyanide | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes bromide | Phanerodontia chrysosporium | ? | - |
? | |
additional information | structural properties | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | enzyme oxidizes ferrocyanide | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | enzyme oxidizes bromide | Phanerodontia chrysosporium OGC101 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, wild-type and mutants R177D, R177E, R177N and R177Q, SDS-PAGE | Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
Mn2+ | mutant E35Q | Phanerodontia chrysosporium | |
17.3 | - |
H2O2 | mutant R177E | Phanerodontia chrysosporium | |
23.2 | - |
H2O2 | mutant R177Q | Phanerodontia chrysosporium | |
25 | - |
Mn2+ | mutant R177Q | Phanerodontia chrysosporium | |
25.5 | - |
Mn2+ | mutant R177E | Phanerodontia chrysosporium | |
28.8 | - |
H2O2 | mutant R177D | Phanerodontia chrysosporium | |
30.2 | - |
Mn2+ | mutant R177D | Phanerodontia chrysosporium | |
34.6 | - |
Mn2+ | mutant R177N | Phanerodontia chrysosporium | |
35.8 | - |
H2O2 | mutant R177N | Phanerodontia chrysosporium | |
184 | - |
Mn2+ | mutant R177A | Phanerodontia chrysosporium | |
190 | - |
H2O2 | mutant R177A | Phanerodontia chrysosporium | |
252 | - |
H2O2 | wild-type MnP | Phanerodontia chrysosporium | |
256 | - |
Mn2+ | wild-type MnP | Phanerodontia chrysosporium | |
262 | - |
Mn2+ | mutant R177K | Phanerodontia chrysosporium | |
273 | - |
H2O2 | mutant R177K | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme iron of the native enzyme is ferric, high-spin and pentacoordinate with a proximal histidine ligand, important catalytic residues in the heme pocket are conserved: proximal His-173 and Asp-242, distal His-46, Arg-42, Asn-80 and Glu-74 | Phanerodontia chrysosporium |