Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-hydroxy acid | activates by chelating and stabilizing Mn3+ rather than activating the enzyme | Dichomitus squalens | |
citrate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
gluconate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
glycolate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
H2O2 | H2O2-dependent | Dichomitus squalens | |
L-malate | activates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
L-Tartrate | stimulates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
Lactate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
Maleate | slightly activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
malonate | stimulates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
additional information | acetate is not an effective chelator | Dichomitus squalens | |
oxalate | activates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
phenylacetate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
phosphate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
succinate | slightly activates, chelates Mn3+ | Dichomitus squalens |
Cloned (Comment) | Organism |
---|---|
MnP1 and MnP2 are encoded by different genes | Dichomitus squalens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
Mn2+ | MnP2 | Dichomitus squalens | |
0.039 | - |
Mn2+ | MnP1 | Dichomitus squalens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Dichomitus squalens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | expression of MnP isoenzymes is dependent on the presence of Mn, expression rather than enzymatic activity is regulated by Mn | Dichomitus squalens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
MnP1 and MnP2, gel filtration | Dichomitus squalens |
48900 | - |
1 * 48000, MnP1, 1 * 48900, MnP2, SDS-PAGE | Dichomitus squalens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Dichomitus squalens | involved in lignin-degradation | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dichomitus squalens | - |
white rot fungus | - |
Dichomitus squalens | - |
2 isoenzymes: MnP1 and MnP2, syn. Polyporus anceps | - |
Dichomitus squalens CBS 432.34 | - |
2 isoenzymes: MnP1 and MnP2, syn. Polyporus anceps | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | neutral carbohydrate content: MnP1: 8.5%, MnP2: 10.3% | Dichomitus squalens |
Purification (Comment) | Organism |
---|---|
148fold purification of MnP1 and 157fold purification of MnP2 | Dichomitus squalens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
174 | - |
MnP1 | Dichomitus squalens |
184 | - |
MnP2 | Dichomitus squalens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Dichomitus squalens | Mn3+ + H2O | - |
? | |
additional information | no oxidation of Co2+ | Dichomitus squalens | ? | - |
? | |
additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Dichomitus squalens | ? | - |
? | |
additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Dichomitus squalens | ? | - |
? | |
additional information | no activity with veratryl alcohol | Dichomitus squalens | ? | - |
? | |
additional information | no oxidation of Fe2+, Cu2+, Zn2+ | Dichomitus squalens | ? | - |
? | |
additional information | no other metal can substitute Mn2+ | Dichomitus squalens | ? | - |
? | |
additional information | enzyme oxidizes 2,6-dimethoxyphenol | Dichomitus squalens | ? | - |
? | |
additional information | no oxidation of Ni2+ | Dichomitus squalens | ? | - |
? | |
additional information | no oxidation of Co2+ | Dichomitus squalens CBS 432.34 | ? | - |
? | |
additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Dichomitus squalens CBS 432.34 | ? | - |
? | |
additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Dichomitus squalens CBS 432.34 | ? | - |
? | |
additional information | no activity with veratryl alcohol | Dichomitus squalens CBS 432.34 | ? | - |
? | |
additional information | no oxidation of Fe2+, Cu2+, Zn2+ | Dichomitus squalens CBS 432.34 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, MnP1, 1 * 48900, MnP2, SDS-PAGE | Dichomitus squalens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay at room temperature | Dichomitus squalens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
MnP1: pI 4.1, MnP2: pI 3.9 | Dichomitus squalens |
4.5 | - |
MnP1 in 50 mM malonate | Dichomitus squalens |
5 | - |
MnP2 in 50 mM malonate | Dichomitus squalens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | enzyme contains a pentacoordinated, essentially high-spin ferric heme | Dichomitus squalens | |
heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Dichomitus squalens |