Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphate | diphosphate proves to be the worst chelator, leading to a specific activity about 4.6% of that obtained with tartrate | Lentinus tigrinus | |
Lactate | lactate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 48.4% of that obtained with tartrate | Lentinus tigrinus | |
malonate | the highest stimulation of 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate, malonate also serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 54.8% of that obtained with tartrate | Lentinus tigrinus | |
oxalate | oxalate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 64.7% of that obtained with tartrate | Lentinus tigrinus | |
Tartrate | the highest rate of Mn3+ formation is obtained with 10 mM tartrate | Lentinus tigrinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
H2O2 | - |
Lentinus tigrinus | |
0.016 | - |
H2O2 | pH 5.5, 45°C | Lentinus tigrinus | |
0.0713 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | - |
Lentinus tigrinus | |
0.0713 | - |
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus | |
0.076 | - |
2,6-dimethoxyphenol | - |
Lentinus tigrinus | |
0.076 | - |
2,6-dimethoxyphenol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
0.124 | - |
Mn2+ | - |
Lentinus tigrinus | |
0.124 | - |
Mn2+ | pH 5.5, 45°C | Lentinus tigrinus | |
0.196 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
Lentinus tigrinus | |
0.196 | - |
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
0.213 | - |
guaiacol | - |
Lentinus tigrinus | |
0.213 | - |
guaiacol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
0.293 | - |
pyrogallol | - |
Lentinus tigrinus | |
0.293 | - |
pyrogallol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
0.56 | - |
veratryl alcohol | - |
Lentinus tigrinus | |
0.56 | - |
veratryl alcohol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
2.3 | - |
guaiacol | - |
Lentinus tigrinus | |
2.3 | - |
guaiacol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus | |
3.74 | - |
pyrogallol | - |
Lentinus tigrinus | |
3.74 | - |
pyrogallol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus | |
6.954 | - |
2,6-dimethoxyphenol | - |
Lentinus tigrinus | |
6.954 | - |
2,6-dimethoxyphenol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Manganese | - |
Lentinus tigrinus | |
Mn2+ | veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+ | Lentinus tigrinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50500 | - |
gel filtration | Lentinus tigrinus |
50500 | - |
determined by gel filtration and SDS-PAGE | Lentinus tigrinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Lentinus tigrinus | - |
Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Lentinus tigrinus CBS 577.79 | - |
Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Lentinus tigrinus 577.79 | - |
Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinus tigrinus | - |
- |
- |
Lentinus tigrinus 577.79 | - |
- |
- |
Lentinus tigrinus CBS 577.79 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation of about 5.3% of the high-mannose type | Lentinus tigrinus |
glycoprotein | the enzyme displays an extent of N-glycosylation of about 5.3% of the high-mannose type | Lentinus tigrinus |
Purification (Comment) | Organism |
---|---|
purification scheme involves ultrafiltration, affinity chromatography on concanavalin-A Sepharose and gel filtration | Lentinus tigrinus |
ultrafiltration, Concanavalin-A Sepharose column chromatography and Superdex 75 gel filtration | Lentinus tigrinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
purification step culture supernatant | Lentinus tigrinus |
40 | - |
culture supernatant, pH 5.5, 45°C | Lentinus tigrinus |
41 | - |
purification step ultrafiltration | Lentinus tigrinus |
169 | - |
purification step chromatography on a concanavalin-A Sepharose column | Lentinus tigrinus |
288 | - |
purification step gel filtration | Lentinus tigrinus |
288 | - |
after 7.2fold purification, pH 5.5, 45°C | Lentinus tigrinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 + H+ | - |
Lentinus tigrinus | ? | - |
r | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ? | - |
Lentinus tigrinus | ? | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ? | - |
Lentinus tigrinus CBS 577.79 | ? | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ? | - |
Lentinus tigrinus 577.79 | ? | - |
? | |
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 | - |
Lentinus tigrinus | ? | - |
? | |
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 + Mn2+ | - |
Lentinus tigrinus | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 | the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate | Lentinus tigrinus | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 | the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate | Lentinus tigrinus CBS 577.79 | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 | the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate | Lentinus tigrinus 577.79 | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + Mn2+ | - |
Lentinus tigrinus | ? | - |
? | |
2,6-dimethoxyphenol + Mn2+ + ? | - |
Lentinus tigrinus | ? | - |
? | |
guaiacol + H2O2 | - |
Lentinus tigrinus | ? | - |
? | |
guaiacol + H2O2 | - |
Lentinus tigrinus CBS 577.79 | ? | - |
? | |
guaiacol + H2O2 | - |
Lentinus tigrinus 577.79 | ? | - |
? | |
guaiacol + H2O2 + Mn2+ | - |
Lentinus tigrinus | ? | - |
? | |
guaiacol + Mn2+ + ? | - |
Lentinus tigrinus | ? | - |
? | |
Mn2+ + H+ + H2O2 | - |
Lentinus tigrinus | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Lentinus tigrinus CBS 577.79 | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Lentinus tigrinus 577.79 | Mn3+ + H2O | - |
? | |
pyrogallol + H2O2 | - |
Lentinus tigrinus | ? | - |
? | |
pyrogallol + H2O2 | - |
Lentinus tigrinus CBS 577.79 | ? | - |
? | |
pyrogallol + H2O2 | - |
Lentinus tigrinus 577.79 | ? | - |
? | |
pyrogallol + H2O2 + Mn2+ | - |
Lentinus tigrinus | ? | - |
? | |
pyrogallol + Mn2+ + ? | - |
Lentinus tigrinus | ? | - |
? | |
veratryl alcohol + H2O2 + Mn2+ | veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+ | Lentinus tigrinus | ? | - |
? | |
veratryl alcohol + Mn2+ + ? | - |
Lentinus tigrinus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Lentinus tigrinus |
monomer | 1 * 50500, SDS-PAGE | Lentinus tigrinus |
Synonyms | Comment | Organism |
---|---|---|
manganese peroxidase | - |
Lentinus tigrinus |
Mn2+: hydrogen peroxide oxidoreductase | - |
Lentinus tigrinus |
Mn2+:hydrogen peroxide oxidoreductase | - |
Lentinus tigrinus |
MnP II | - |
Lentinus tigrinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Lentinus tigrinus |
45 | - |
optimum temperature for the oxidation of Mn2+ and DMOP | Lentinus tigrinus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | the enzyme shows relative activities of 34, 50, and 47% at 10, 40, and 60°C, respectively | Lentinus tigrinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme is rather unstable at 50, 60, and 70°C, resulting in half-lives of 660, 105, and 41 s, respectively | Lentinus tigrinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
veratryl alcohol | - |
Lentinus tigrinus | |
0.45 | - |
veratryl alcohol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
0.74 | - |
guaiacol | - |
Lentinus tigrinus | |
0.74 | - |
guaiacol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus | |
1.87 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+ | - |
Lentinus tigrinus | |
1.87 | - |
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus | |
5.67 | - |
2,6-dimethoxyphenol | - |
Lentinus tigrinus | |
5.67 | - |
2,6-dimethoxyphenol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus | |
8.92 | - |
guaiacol | - |
Lentinus tigrinus | |
8.92 | - |
guaiacol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
9.98 | - |
pyrogallol | - |
Lentinus tigrinus | |
9.98 | - |
pyrogallol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C | Lentinus tigrinus | |
20.2 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
Lentinus tigrinus | |
20.2 | - |
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
20.95 | - |
pyrogallol | - |
Lentinus tigrinus | |
20.95 | - |
pyrogallol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
26.26 | - |
2,6-dimethoxyphenol | - |
Lentinus tigrinus | |
26.26 | - |
2,6-dimethoxyphenol | in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C | Lentinus tigrinus | |
121 | - |
H2O2 | - |
Lentinus tigrinus | |
121 | - |
H2O2 | with 1.5 mM Mn2+ as cosubstrate, pH 5.5, 45°C | Lentinus tigrinus | |
242.4 | - |
Mn2+ | - |
Lentinus tigrinus | |
242.4 | - |
Mn2+ | with 0.2 mM H2O2 as cosubstrate, pH 5.5, 45°C | Lentinus tigrinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
optimal pH for the Mn-dependent oxidation of veratryl alcohol | Lentinus tigrinus |
3 | - |
optimum pH for the Mn2+-dependent oxidation of veratryl alehyde | Lentinus tigrinus |
3.5 | 4.5 | pH optimum for Mn2+-independent DMOP oxidation | Lentinus tigrinus |
4 | 4.5 | pH optimum for Mn2+-dependent DMOP oxidation | Lentinus tigrinus |
5.5 | - |
- |
Lentinus tigrinus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | Mn2+ oxidation shows an optimum in the range 5.5-5.75 and negligible activity at pH values below 3.5 and above 6.5 | Lentinus tigrinus |
3.5 | 4.5 | optima for Mn2+-independent 2,6-dimethoxyphenol oxidation | Lentinus tigrinus |
4 | 4.5 | optima for Mn2+-dependent 2,6-dimethoxyphenol oxidation | Lentinus tigrinus |
5.5 | 5.8 | the pH-activity profile for Mn2+ oxidation shows an optimum in the range 5.5 - 5.75 and negligible activity at pHs below 3.5 and above 6.5 | Lentinus tigrinus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.5 | the enzyme shows a half-life of 8.8 h at pH 3.5, the half-lives of MnP activity at pH 4.5, 5.0, and 5.5 are 89.5, 99, and 101 h, respectively | Lentinus tigrinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the heme content is 0.96 mol per mole of the enzyme | Lentinus tigrinus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lentinus tigrinus | - |
- |
4.07 |
Lentinus tigrinus | isoelectric focusing | - |
4.1 |