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Literature summary for 1.11.1.13 extracted from

  • Thamvithayakorn, P.; Phosri, C.; Pisutpaisal, N.; Krajangsang, S.; Whalley, A.J.S.; Suwannasai, N.
    Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33 (2019), 3 Biotech, 9, 417 .
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2 Pseudolagarobasidium sp. PP17-33
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 2 Mn(III) + 2 H2O
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 ?

Organism

Organism UniProt Comment Textmining
Pseudolagarobasidium sp. PP17-33
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 fruiting bodies of basidiomycete fungi found on decayed or fallen wood are collected from various parts of Thailand, e.g. provinces of Chaiyaphum, Nakhon Phanom, Sakon Nakhon and Udon Thani
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Source Tissue

Source Tissue Comment Organism Textmining
fruit body
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 Pseudolagarobasidium sp. PP17-33
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additional information the white-rot fungus Pseudolagarobasidium sp. PP17-33 can be utilized for valuable manganese peroxidase (MnP) enzyme production from oil palm decanter cake. Optimization to enhance the production of enzymes through solid-state fermentation is performed using Plackett-Burman design and response surface methodology. The highest observed MnP is 5.156 U/gds, which enhanceds yield by 1.94fold from the non-optimization. The optimized medium (mg/g of OPDC) consists of 0.852 mg CuSO4/5H2O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH2PO4, 1.5 mg MgSO4/7H2O, 0.01 mg FeSO4/7H2O, 0.15 mg MnSO4/H2O, 0.01 mg ZnSO4/7H2O and 0.3 mg Tween 80, pH 5.0, when incubated at 30°C for 7 days. The most significant variables of MnP productions are CuSO4/5H2O and glucose concentrations Pseudolagarobasidium sp. PP17-33
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 screening of enzyme activity duirng optimization of enzyme production method from Pseudolagarobasidium sp. PP17-33, overview Pseudolagarobasidium sp. PP17-33

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2
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 Pseudolagarobasidium sp. PP17-33 2 Mn(III) + 2 H2O
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 ?
additional information MnP activity is determined by monitoring the oxidation of 2,6-dimethoxyphenol (DMP) as the oxidation of Mn2+ to Mn3+ by following the formation of the Mn3+-tartrate complex at 469 nm Pseudolagarobasidium sp. PP17-33 ?
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Synonyms

Synonyms Comment Organism
MnP
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 Pseudolagarobasidium sp. PP17-33

Cofactor

Cofactor Comment Organism Structure
heme
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 Pseudolagarobasidium sp. PP17-33