Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ferulic acid | - |
Cerrena unicolor | |
gallic acid | - |
Cerrena unicolor | |
Tween 80 | - |
Cerrena unicolor |
General Stability | Organism |
---|---|
Ca2+ reduces the enzyme stability by 40% at 10 mM | Cerrena unicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 30% inhibition at 10 mM, Ca2+ reduces the enzyme stability by 40% | Cerrena unicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0321 | - |
H2O2 | pH 4.8, 30°C | Cerrena unicolor | |
0.0467 | - |
Mn(II) | pH 4.8, 30°C | Cerrena unicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | manganese peroxidase (MnP) is an extracellular glycosylated heme protein | Cerrena unicolor | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, enzyme-bound, 40% inhibition at 10 mM | Cerrena unicolor | |
Fe2+ | in the heme group | Cerrena unicolor | |
Mg2+ | slightly activating at 10 mM | Cerrena unicolor | |
Mn2+ | 20% activating at 10 mM | Cerrena unicolor | |
additional information | poor effects by 10 mM of Zn2+, Na+, and K+ | Cerrena unicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | Cerrena unicolor | - |
2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | Cerrena unicolor BBP6 | - |
2 Mn(III) + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerrena unicolor | A0A7D5FUQ6 | - |
- |
Cerrena unicolor BBP6 | A0A7D5FUQ6 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 6.9fold by anion exchange chromatography | Cerrena unicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
112.4 | - |
purified enzyme, pH 4.8, 30°C | Cerrena unicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | - |
Cerrena unicolor | 2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | - |
Cerrena unicolor BBP6 | 2 Mn(III) + 2 H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H+ + H2O2 | - |
Cerrena unicolor | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | - |
Cerrena unicolor | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
brilliant blue R + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
bromophenol blue + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
bromophenol blue + H2O2 | dye decolorization | Cerrena unicolor BBP6 | ? | - |
? | |
Congo red + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
crystal violet + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
crystal violet + H2O2 | dye decolorization | Cerrena unicolor BBP6 | ? | - |
? | |
guaiacol + 2 H+ + H2O2 | - |
Cerrena unicolor | oxidized guaiacol + 2 H2O | - |
? | |
methyl orange + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
additional information | evaluation of the dye decolorization ability of the purified enzyme, MnP-BBP6, the enzyme is used to decolorize different types of synthetic dyes including RBBR, Congo red (CR), brilliant blue R (BBR), methyl orange (MO), bromophenol blue (BPB), and crystal violet (CV). Denim bleaching by the purified MnP-BBP6 | Cerrena unicolor | ? | - |
- |
|
additional information | evaluation of the dye decolorization ability of the purified enzyme, MnP-BBP6, the enzyme is used to decolorize different types of synthetic dyes including RBBR, Congo red (CR), brilliant blue R (BBR), methyl orange (MO), bromophenol blue (BPB), and crystal violet (CV). Denim bleaching by the purified MnP-BBP6 | Cerrena unicolor BBP6 | ? | - |
- |
|
Remazol Brilliant Blue R + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
Remazol Brilliant Blue R + H2O2 | dye decolorization | Cerrena unicolor BBP6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Cerrena unicolor |
Synonyms | Comment | Organism |
---|---|---|
MnP | - |
Cerrena unicolor |
MnP-BBP6 | - |
Cerrena unicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Cerrena unicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme, pH 4.8, 90% activity remaining | Cerrena unicolor |
40 | - |
purified enzyme, pH 4.8, completely stable at | Cerrena unicolor |
60 | - |
purified enzyme, pH 4.8, 75% activity remaining | Cerrena unicolor |
80 | - |
purified enzyme, pH 4.8, inactivation | Cerrena unicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Cerrena unicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.5 | over 50% of maximal activity within this range | Cerrena unicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | purified enzyme, 30°C, full stability at pH 4.5, below 10% activity remaining at pH 3.0 and pH 7.5, 90% at pH 4.0, 80% at pH 5.0, and 30% at pH 6.0 | Cerrena unicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cerrena unicolor |