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Literature summary for 1.11.1.14 extracted from

  • Rekik, H.; Nadia, Z.J.; Bejar, W.; Kourdali, S.; Belhoul, M.; Hmidi, M.; Benkiar, A.; Badis, A.; Sallem, N.; Bejar, S.; Jaouadi, B.
    Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9 (2015), Int. J. Biol. Macromol., 73, 253-263 .
    View publication on PubMed
Search for more literature for 1.11.1.14

Activating Compound

Activating Compound Comment Organism Structure
sodium carbonate 50 mM, 125% of initial activity Streptomyces griseosporeus
tetraacetylethylenediamine 25 mM, 121% of initial activity Streptomyces griseosporeus
Triton X-100 5% w/v, 123% of initial activity Streptomyces griseosporeus

Application

Application Comment Organism
degradation enzyme shows marked dye-decolorization efficiency and stability toward denaturing, oxidizing, and bleaching agents, and compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C Streptomyces griseosporeus

Inhibitors

Inhibitors Comment Organism Structure
SDS 1% w/v, 72% residual activity Streptomyces griseosporeus
sodium perborate 5% w/v, 71% residual activity Streptomyces griseosporeus
Urea 1% w/v, 85% residual activity Streptomyces griseosporeus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 2,4-Dichlorophenol pH 8.5, 60°C Streptomyces griseosporeus
0.46
-
 o-Dianisidine pH 8.5, 60°C Streptomyces griseosporeus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces griseosporeus
-
-

Organism

Organism UniProt Comment Textmining
Streptomyces griseosporeus
-
-
-
Streptomyces griseosporeus SN9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification from culture supernatant Streptomyces griseosporeus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.75
-
 substrate 2,4-dichlorophenol, pH 8.5, temperature not specified in the publication Streptomyces griseosporeus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 guaiacol + H2O2 27% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
2 guaiacol + H2O2 27% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 92% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 92% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 ? + H2O
-
 ?
2,4,6-trichlorophenol + H2O2 63% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
2,4,6-trichlorophenol + H2O2 63% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 ? + H2O
-
 ?
2,4-dichlorophenol + H2O2 best substrate Streptomyces griseosporeus ? + H2O
-
 ?
4-chlorophenol + H2O2 45% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
humic acid + H2O2
-
 Streptomyces griseosporeus ? + H2O
-
 ?
humic acid + H2O2
-
 Streptomyces griseosporeus SN9 ? + H2O
-
 ?
o-dianisidine + H2O2 76% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
o-dianisidine + H2O2 76% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 ? + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Streptomyces griseosporeus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces griseosporeus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 2,4-Dichlorophenol pH 8.5, 60°C Streptomyces griseosporeus
1.16
-
 o-Dianisidine pH 8.5, 60°C Streptomyces griseosporeus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Streptomyces griseosporeus

Cofactor

Cofactor Comment Organism Structure
heme spectrum shows a heme peak at 409 nm Streptomyces griseosporeus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.57
-
 2,4-Dichlorophenol pH 8.5, 60°C Streptomyces griseosporeus
2.53
-
 o-Dianisidine pH 8.5, 60°C Streptomyces griseosporeus