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Literature summary for 1.11.1.14 extracted from

  • Kiran, M.R.; Rathour, R.K.; Bhatia, R.K.; Rana, D.S.; Bhatt, A.K.; Thakur, N.
    Fabrication of thermostable and reusable nanobiocatalyst for dye decolourization by immobilization of lignin peroxidase on graphene oxide functionalized MnFe2O4 superparamagnetic nanoparticles (2020), Biores. Technol., 317, 124020 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information partially purified lignin peroxidase (LiP) from Pseudomonas fluorescens strain LiP-RL5 is immobilized on graphene oxide functionalized MnFe2O4 nanoparticles (10 nm, synthesized by sol-gel auto-combustion) to fabricate a new hyperactive and thermostable nanobiocatalyst, immobilization method evaluation, overview. Immobilized LiP is quite stable at 50°C with the half-life of 14 h and shows higher tolerance towards various metal ions and solvents than free LiP. Immobilized LiP retains 50% of enzyme activity even after nine consecutive runs. When tested against various textile dyes, the immobilized LiP is effective with higher dye decolourization efficiency (up to 88%) within 1 h of incubation at 30°C Pseudomonas fluorescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas fluorescens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+
-
Pseudomonas fluorescens
Ca2+
-
Pseudomonas fluorescens
Cd2+
-
Pseudomonas fluorescens
Fe3+
-
Pseudomonas fluorescens
Hg2+
-
Pseudomonas fluorescens
K+
-
Pseudomonas fluorescens
Mg2+
-
Pseudomonas fluorescens
Mn2+
-
Pseudomonas fluorescens
Na+
-
Pseudomonas fluorescens
NH4+
-
Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens LiP-RL5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme partially by ammonium sulfate fractionation and dialysis Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.79
-
partially purified enzyme, pH 5.5, 30°C Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 veratryl alcohol + H2O2
-
Pseudomonas fluorescens 2 veratryl aldehyde + 2 H2O
-
?
2 veratryl alcohol + H2O2
-
Pseudomonas fluorescens LiP-RL5 2 veratryl aldehyde + 2 H2O
-
?
bromophenol blue + H2O2 decolorization of a textile dye Pseudomonas fluorescens ?
-
?
bromophenol blue + H2O2 decolorization of a textile dye Pseudomonas fluorescens LiP-RL5 ?
-
?
coomassie brilliant blue + H2O2 decolorization of a textile dye Pseudomonas fluorescens ?
-
?
coomassie brilliant blue + H2O2 decolorization of a textile dye Pseudomonas fluorescens LiP-RL5 ?
-
?
methylene blue + H2O2 decolorization of a textile dye Pseudomonas fluorescens ?
-
?
methylene blue + H2O2 decolorization of a textile dye Pseudomonas fluorescens LiP-RL5 ?
-
?
nigrosine + H2O2 decolorization of a textile dye Pseudomonas fluorescens ?
-
?
nigrosine + H2O2 decolorization of a textile dye Pseudomonas fluorescens LiP-RL5 ?
-
?

Synonyms

Synonyms Comment Organism
LIP
-
Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
heme
-
Pseudomonas fluorescens