KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
vanillylidenacetone | presence of Mn2+, isoenzyme MP-1, pH 5.0 | Pleurotus eryngii | |
0.005 | - |
vanillylidenacetone | presence of Mn2+, isoenzyme MP-2, pH 5.0 | Pleurotus eryngii | |
0.006 | - |
H2O2 | cosubstrate Mn2+, isoenzyme MP-1, pH 5.0 | Pleurotus eryngii | |
0.01 | - |
H2O2 | cosubstrate Mn2+, isoenzyme MP-2, pH 5.0 | Pleurotus eryngii | |
0.01 | - |
2,6-dimethoxyphenol | presence of Mn2+, isoenzyme MP-1, pH 5.0 | Pleurotus eryngii | |
0.01 | - |
2,6-dimethoxyphenol | presence of Mn2+, isoenzyme MP-2, pH 5.0 | Pleurotus eryngii | |
0.015 | - |
Mn2+ | cosubstrate dimethoxyphenol, isoenzyme MP-1, pH 5.0 | Pleurotus eryngii | |
0.015 | - |
Mn2+ | cosubstrate dimethoxyphenol, isoenzyme MP-2, pH 5.0 | Pleurotus eryngii | |
0.02 | - |
Mn2+ | cosubstrate H2O2, isoenzyme MP-1, pH 5.0 | Pleurotus eryngii | |
0.02 | - |
Mn2+ | cosubstrate H2O2, isoenzyme MP-2, pH 5.0 | Pleurotus eryngii | |
0.16 | - |
2,6-dimethoxyphenol | absence of Mn2+, isoenzyme MP-1, pH 3.0 | Pleurotus eryngii | |
0.25 | - |
2,6-dimethoxyphenol | absence of Mn2+, isoenzyme MP-2, pH 3.0 | Pleurotus eryngii | |
3 | - |
veratryl alcohol | absence of Mn2+, isoenzyme MP-2, pH 3.0 | Pleurotus eryngii | |
3.5 | - |
veratryl alcohol | absence of Mn2+, isoenzyme MP-1, pH 3.0 | Pleurotus eryngii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE, both isoenzyme MP-1 and MP-2 | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | - |
isoenzymes MP-1 and MP-2, addition of Mn2+ to growth medium represses enzyme activity | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | MP-1, up to 5% carbohydrate content, MP-2, up to 7% carbohydrate | Pleurotus eryngii |
Purification (Comment) | Organism |
---|---|
- |
Pleurotus eryngii |
Storage Stability | Organism |
---|---|
-20°C, stable | Pleurotus eryngii |
4°C, stable for at least 72h | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2,6-dimethoxyphenol + 2 H2O2 | Mn2+-dependent and independent activity | Pleurotus eryngii | coerulignone + 2 H2O | - |
? | |
Mn2+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | - |
? | |
NADH + H2O2 | - |
Pleurotus eryngii | NAD+ + H2O | - |
? | |
phenol red + H2O2 | Mn2+-dependent activity | Pleurotus eryngii | oxidized phenol red + H2O | - |
? | |
vanillylidenacetone + H2O2 | Mn2+-dependent activity | Pleurotus eryngii | ? + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | Mn2+-independent activity | Pleurotus eryngii | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE, both isoenzyme MP-1 and MP-2 | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
oxidation of 2,6-dimethoxyphenol, absence of Mn2+ | Pleurotus eryngii |
4 | - |
oxidation of 2,6-dimethoxyphenol, presence of Mn2+ | Pleurotus eryngii |
5 | - |
oxidation of Mn2+ | Pleurotus eryngii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pleurotus eryngii | isoelectric focusing, isoenzyme MP-2 | - |
3.65 |
Pleurotus eryngii | isoelectric focusing, isoenzyme MP-2 | - |
3.75 |