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Literature summary for 1.11.1.16 extracted from

  • Martinez, M.J.; Ruiz-Duenas, F.J.; Guillen, F.; Martinez, A.T.
    Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii (1996), Eur. J. Biochem., 237, 424-432.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
vanillylidenacetone presence of Mn2+, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
0.005
-
vanillylidenacetone presence of Mn2+, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
0.006
-
H2O2 cosubstrate Mn2+, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
0.01
-
H2O2 cosubstrate Mn2+, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
0.01
-
2,6-dimethoxyphenol presence of Mn2+, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
0.01
-
2,6-dimethoxyphenol presence of Mn2+, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
0.015
-
Mn2+ cosubstrate dimethoxyphenol, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
0.015
-
Mn2+ cosubstrate dimethoxyphenol, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
0.02
-
Mn2+ cosubstrate H2O2, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
0.02
-
Mn2+ cosubstrate H2O2, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
0.16
-
2,6-dimethoxyphenol absence of Mn2+, isoenzyme MP-1, pH 3.0 Pleurotus eryngii
0.25
-
2,6-dimethoxyphenol absence of Mn2+, isoenzyme MP-2, pH 3.0 Pleurotus eryngii
3
-
veratryl alcohol absence of Mn2+, isoenzyme MP-2, pH 3.0 Pleurotus eryngii
3.5
-
veratryl alcohol absence of Mn2+, isoenzyme MP-1, pH 3.0 Pleurotus eryngii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE, both isoenzyme MP-1 and MP-2 Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii
-
isoenzymes MP-1 and MP-2, addition of Mn2+ to growth medium represses enzyme activity
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein MP-1, up to 5% carbohydrate content, MP-2, up to 7% carbohydrate Pleurotus eryngii

Purification (Commentary)

Purification (Comment) Organism
-
Pleurotus eryngii

Storage Stability

Storage Stability Organism
-20°C, stable Pleurotus eryngii
4°C, stable for at least 72h Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2,6-dimethoxyphenol + 2 H2O2 Mn2+-dependent and independent activity Pleurotus eryngii coerulignone + 2 H2O
-
?
Mn2+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O
-
?
NADH + H2O2
-
Pleurotus eryngii NAD+ + H2O
-
?
phenol red + H2O2 Mn2+-dependent activity Pleurotus eryngii oxidized phenol red + H2O
-
?
vanillylidenacetone + H2O2 Mn2+-dependent activity Pleurotus eryngii ? + H2O
-
?
veratryl alcohol + H2O2 + H+ Mn2+-independent activity Pleurotus eryngii veratraldehyde + H2O
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE, both isoenzyme MP-1 and MP-2 Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
oxidation of 2,6-dimethoxyphenol, absence of Mn2+ Pleurotus eryngii
4
-
oxidation of 2,6-dimethoxyphenol, presence of Mn2+ Pleurotus eryngii
5
-
oxidation of Mn2+ Pleurotus eryngii

pI Value

Organism Comment pI Value Maximum pI Value
Pleurotus eryngii isoelectric focusing, isoenzyme MP-2
-
3.65
Pleurotus eryngii isoelectric focusing, isoenzyme MP-2
-
3.75