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Literature summary for 1.11.1.16 extracted from

  • Siddiqui, K.S.; Ertan, H.; Charlton, T.; Poljak, A.; Daud Khaled, A.K.; Yang, X.; Marshall, G.; Cavicchioli, R.
    Versatile peroxidase degradation of humic substances use of isothermal titration calorimetry to assess kinetics, and applications to industrial wastes (2014), J. Biotechnol., 178, 1-11 .
    View publication on PubMed

Application

Application Comment Organism
degradation the allosteric behaviour of the VP enzyme promotes a high level of regulation of activity during the breakdown of model and industrial ligninolytic substrates, such as effluent from a pulp and paper plant, and fouled membrane solids extracted from a ground water treatment membrane Bjerkandera adusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
humic acid Hill coefficient 1.2, absence of Mn2+, Km value 0.08 g/l, pH 3.5, 25°C Bjerkandera adusta
additional information
-
humic acid Hill coefficient 2.8, presence of Mn2+, Km value 0.005 g/l, pH 3.5, 25°C Bjerkandera adusta
additional information
-
fulvic acid Hill coefficient 3.0, presence of Mn2+, Km value 0.009 g/l, pH 3.5, 25°C Bjerkandera adusta
additional information
-
fulvic acid Hill coefficient 3.6, absence of Mn2+, Km value 0.044 g/l, pH 3.5, 25°C Bjerkandera adusta

Organism

Organism UniProt Comment Textmining
Bjerkandera adusta
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Bjerkandera adusta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fulvic acid + H2O2
-
Bjerkandera adusta ? + H2O
-
?
humic acid + H2O2
-
Bjerkandera adusta ? + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
fulvic acid Hill coefficient 3.6, absence of Mn2+, pH 3.5, 25°C Bjerkandera adusta
0.02
-
humic acid Hill coefficient 2.8, presence of Mn2+, pH 3.5, 25°C Bjerkandera adusta
0.021
-
humic acid Hill coefficient 1.2, absence of Mn2+, pH 3.5, 25°C Bjerkandera adusta
0.045
-
fulvic acid Hill coefficient 3.0, presence of Mn2+, pH 3.5, 25°C Bjerkandera adusta

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
humic acid Hill coefficient 1.2, absence of Mn2+, kcat/Km value 15.5 g/l/min, pH 3.5, 25°C Bjerkandera adusta
additional information
-
humic acid Hill coefficient 2.8, presence of Mn2+, kcat/Km value 240 g/l/min, pH 3.5, 25°C Bjerkandera adusta
additional information
-
fulvic acid Hill coefficient 3.0, presence of Mn2+, kcat/Km value 300 g/l/min, pH 3.5, 25°C Bjerkandera adusta
additional information
-
fulvic acid Hill coefficient 3.6, absence of Mn2+, kcat/Km value 25 g/l/min, pH 3.5, 25°C Bjerkandera adusta