General Stability | Organism |
---|---|
dialysis of the enzyme preparation against 1 mM EDTA in 0.1 M citrate-phosphate buffer (pH 3.8) results in loss of enzymic activity. Incubation with vanadium, does not restore the enzymic activity. Also various other metal ions: Zn(II), Fe(II), Cu(II) and Mn(II) are ineffective in the reactivation of the preparation | Kitasatospora aureofaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
F- | - |
Kitasatospora aureofaciens | |
additional information | not inhibited by azide or cyanide. Excess bromide or chloride has no effect on its brominating activity | Kitasatospora aureofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
H2O2 | - |
Kitasatospora aureofaciens | |
20 | - |
Br- | - |
Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Kitasatospora aureofaciens |
65000 | - |
non-denaturing PAGE | Kitasatospora aureofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
ATCC 10762 | - |
Purification (Comment) | Organism |
---|---|
- |
Kitasatospora aureofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Kitasatospora aureofaciens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Kitasatospora aureofaciens |
Synonyms | Comment | Organism |
---|---|---|
non-haem bromoperoxidase | - |
Kitasatospora aureofaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
activity remains constant for at least 4 h | Kitasatospora aureofaciens |
70 | - |
slow inactivation | Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.3 | - |
the pH optimum is independent of H2O2 and KBr when concentrations ranging from 5 to 100 mM | Kitasatospora aureofaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme does not contain haem | Kitasatospora aureofaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
F- | - |
Kitasatospora aureofaciens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Kitasatospora aureofaciens | isoelectric focusing, pH-range 3-10 | - |
3.5 |