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Literature summary for 1.11.1.18 extracted from
- Purification, properties and immunological detection of a bromoperoxidase-catalase from Streptomyces venezuelae and from a chloramphenicol-nonproducing mutant (1989), J. Gen. Microbiol., 135, 2493-2502.
General Stability
| General Stability | Organism |
|---|---|
| after treatment with a mixture of chloroform/ethanol the enzyme still shows 65% of its initial brominating activity | Streptomyces venezuelae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Amino-1,2,4-triazole | 45% inhibition at 5 mM, 90% inhibition at 40 mM | Streptomyces venezuelae |  |
| hydroxylamine | 0.05 mM, 50% inhibition of brominating activity | Streptomyces venezuelae | |
| potassium cyanide | 0.05 mM, complete inhibition of bromoperoxidase-catalase activity | Streptomyces venezuelae | |
| Sodium azide | 0.05 mM, complete inhibition of bromoperoxidase-catalase activity | Streptomyces venezuelae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
2 * 61000, SDS-PAGE | Streptomyces venezuelae |
| 127000 | 136000 | gel filtration | Streptomyces venezuelae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces venezuelae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces venezuelae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.77 | - |
bromoperoxidase activity | Streptomyces venezuelae |
| 963 | - |
catalase activity | Streptomyces venezuelae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Streptomyces venezuelae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 61000, SDS-PAGE | Streptomyces venezuelae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bromoperoxidase-catalase | - |
Streptomyces venezuelae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.75 | - |
bromination of 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | Streptomyces venezuelae |
| 7 | 8.5 | catalase activity | Streptomyces venezuelae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces venezuelae | isoelectric focusing, pH-range: 3.5-9.5 | - |
4.5 |
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