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Literature summary for 1.11.1.19 extracted from

  • Brissos, V.; Tavares, D.; Sousa, A.; Robalo, M.; Martins, L.
    Engineering a bacterial DyP-type peroxidase for enhanced oxidation of lignin-related phenolics at alkaline pH (2017), ACS Catal., 7, 3454-3465 .
No PubMed abstract available
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
E188K the mutant shows 3-4fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
E188K/A142V the mutant shows 2fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
E188K/A142V/H125Y the mutant shows a 100fold enhanced catalytic efficiency (kcat/Km) for 2,6-dimethoxyphenol and 2fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
E188K/H125R the mutant shows 6fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
H125R the mutant shows 3-4fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
H2O2
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 2,6-dimethoxyphenol mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
0.042
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
0.044
-
 H2O2 mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
0.047
-
 H2O2 mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
0.058
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
0.06
-
 2,6-dimethoxyphenol mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
0.06
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
0.07
-
 2,6-dimethoxyphenol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
0.08
-
 2,6-dimethoxyphenol mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
0.16
-
 H2O2 mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
0.2
-
 H2O2 mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
0.2
-
 H2O2 mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
0.22
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
0.22
-
 H2O2 mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
0.25
-
 2,6-dimethoxyphenol mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
0.26
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
0.28
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
0.5
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
2.5
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida MET94
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1,2-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
1,4-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1,4-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
2 1,2-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida 4a,10a-dihydrophenazine-2,3-diamine + H2O
-
 ?
2 2,5-diaminobenzenesulfonic acid + H2O2 + H+
-
 Pseudomonas putida 2,7-diaminophenazine-1,6-disulfonic acid + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pseudomonas putida ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pseudomonas putida MET94 ?
-
 ?
2,5-diaminobenzenesulfonic acid + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
3 1,4-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida (3E,6E)-3,6-bis[(4-aminophenyl)imino]cyclohexa-1,4-diene-1,4-diamine + H2O
-
 ?
3 N1-phenylbenzene-1,4-diamine + H2O2 + H+
-
 Pseudomonas putida (3E,6E)-3,6-bis[(4-anilinophenyl)imino]-N1-phenylcyclohexa-1,4-diene-1,4-diamine + H2O
-
 ?
4-aminodiphenylamine + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
acetosyringone + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
guaiacol + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
guaiacol + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
lignin + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
methyl syringate + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
syringaldehyde + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Pseudomonas putida
DyP
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 2,6-dimethoxyphenol mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
0.03
-
 2,6-dimethoxyphenol mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
0.05
-
 2,6-dimethoxyphenol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
0.09
-
 2,6-dimethoxyphenol mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.8
-
 2,6-dimethoxyphenol mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
3.6
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
4.4
-
 H2O2 mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
4.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
6
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
15
-
 H2O2 mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
15
-
 H2O2 mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
23
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
23
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
26
-
 H2O2 mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
26
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
69
-
 H2O2 mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
69
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
128
-
 H2O2 mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
128
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
heme
-
 Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
 methyl syringate wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
0.4
-
 2,6-dimethoxyphenol mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
0.45
-
 1,2-phenylenediamine wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
0.6
-
 2,6-dimethoxyphenol mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
0.7
-
 2,6-dimethoxyphenol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.1
-
 1,2-phenylenediamine mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
1.2
-
 guaiacol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.2
-
 syringaldehyde wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.5
-
 2,6-dimethoxyphenol mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.6
-
 lignin wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
2.5
-
 1,4-phenylenediamine wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
2.6
-
 acetosyringone wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
2.9
-
 2,5-diaminobenzenesulfonic acid wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
3.8
-
 2,5-diaminobenzenesulfonic acid mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
4.1
-
 lignin mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
5
-
 acetosyringone mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
7.2
-
 2,6-dimethoxyphenol mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
14
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
14
-
 methyl syringate mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
17
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
18
-
 1,4-phenylenediamine mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
22
-
 guaiacol mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
33
-
 syringaldehyde mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
52
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
60
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
90
-
 H2O2 mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
90
-
 H2O2 mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
90
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
110
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
120
-
 H2O2 mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
220
-
 4-aminodiphenylamine wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
250
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
340
-
 H2O2 mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
350
-
 H2O2 mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
380
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
420
-
 4-aminodiphenylamine mutant enzyme E188K/A142V/H125Y, at pH 5.0 and 25°C Pseudomonas putida
640
-
 H2O2 mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida