Cloned (Comment) | Organism |
---|---|
expressed in Trichoderma reesei | Pleurotus sapidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Pleurotus sapidus | |
0.099 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 3.8 and 30°C | Pleurotus sapidus | |
0.713 | - |
guaiacol | at pH 4.5 and 30°C | Pleurotus sapidus | |
1.227 | - |
2,6-dimethoxyphenol | at pH 4.5 and 30°C | Pleurotus sapidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration | Pleurotus sapidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Reactive Blue 5 + H2O2 | Pleurotus sapidus | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
Reactive Blue 5 + H2O2 | Pleurotus sapidus DSM 8266 | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus sapidus | A0A0F7VJ89 | - |
- |
Pleurotus sapidus DSM 8266 | A0A0F7VJ89 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pleurotus sapidus |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography and SP Sepharose 200 column chromatography | Pleurotus sapidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pleurotus sapidus | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pleurotus sapidus DSM 8266 | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Pleurotus sapidus DSM 8266 | ? | - |
? | |
annatto + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
beta-carotene + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Pleurotus sapidus DSM 8266 | ? | - |
? | |
additional information | the enzyme has an oxidase function in addition to a peroxidase activity | Pleurotus sapidus | ? | - |
? | |
additional information | the enzyme has an oxidase function in addition to a peroxidase activity | Pleurotus sapidus DSM 8266 | ? | - |
? | |
Reactive Blue 5 + H2O2 | - |
Pleurotus sapidus | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
Reactive Blue 5 + H2O2 | - |
Pleurotus sapidus DSM 8266 | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 57100, calculated from amino acid sequence | Pleurotus sapidus |
homodimer | 2 * 57600, SDS-PAGE | Pleurotus sapidus |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Pleurotus sapidus |
DyP | - |
Pleurotus sapidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 70 | after 5 min incubation, respectively, the enzyme shows 100% activity between 15 and 25°C, about 85% activity at 30-35°C, about 75% activity at 40-45°C, about 62% activity at 50°C, and less than 30% activity at 55°C and above | Pleurotus sapidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Pleurotus sapidus | |
60 | - |
2,6-dimethoxyphenol | at pH 4.5 and 30°C | Pleurotus sapidus | |
74 | - |
guaiacol | at pH 4.5 and 30°C | Pleurotus sapidus | |
375 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 3.8 and 30°C | Pleurotus sapidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | - |
Pleurotus sapidus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pleurotus sapidus | calculated from amino acid sequence | - |
5.7 |
Pleurotus sapidus | isoelectric focusing | - |
6.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49 | - |
2,6-dimethoxyphenol | at pH 4.5 and 30°C | Pleurotus sapidus | |
100 | - |
guaiacol | at pH 4.5 and 30°C | Pleurotus sapidus | |
750 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Pleurotus sapidus | |
3800 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 3.8 and 30°C | Pleurotus sapidus |