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Literature summary for 1.11.1.19 extracted from
- New dye-decolorizing peroxidases from Bacillus subtilis and Pseudomonas putida MET94 Towards biotechnological applications (2014), Appl. Microbiol. Biotechnol., 98, 2053-2065 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) Star cells | Bacillus subtilis |
| expressed in Escherichia coli BL21(DE3) Star cells | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Bacillus subtilis |  |
| H2O2 | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
H2O2 | with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 4.0 and 30°C | Bacillus subtilis | |
| 0.03 | - |
Acid Blue 62 | at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.035 | - |
guaiacol | at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.035 | - |
acetosyringone | at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.04 | - |
Reactive Blue 5 | at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.048 | - |
syringaldehyde | at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.079 | - |
H2O2 | with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.123 | - |
Mn2+ | at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.157 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Bacillus subtilis | |
| 0.166 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 30°C | Bacillus subtilis | |
| 0.32 | - |
Mordant black 9 | at pH 5.0 and 30°C | Pseudomonas putida | |
| 0.385 | - |
Mordant black 9 | at pH 4.0 and 30°C | Bacillus subtilis | |
| 0.444 | - |
Acid Blue 62 | at pH 4.0 and 30°C | Bacillus subtilis | |
| 0.499 | - |
guaiacol | at pH 4.0 and 30°C | Bacillus subtilis | |
| 2.5 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 5.0 and 30°C | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Bacillus subtilis |
| 120000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Reactive Blue 5 + H2O2 | Bacillus subtilis | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
| Reactive Blue 5 + H2O2 | Pseudomonas putida | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
| Reactive Blue 5 + H2O2 | Bacillus subtilis 168 | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
| Reactive Blue 5 + H2O2 | Pseudomonas putida MET94 | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 168 | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida MET94 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q Sepharose column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
| SP-Sepharose column chromatography Mono S column chromatography, and Superdex 75 gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Bacillus subtilis | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pseudomonas putida | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Bacillus subtilis 168 | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pseudomonas putida MET94 | ? | - |
? | |
| acetosyringone + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| acetosyringone + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| acetosyringone + H2O2 + H+ | - |
Bacillus subtilis 168 | ? | - |
? | |
| acetosyringone + H2O2 + H+ | - |
Pseudomonas putida MET94 | ? | - |
? | |
| Acid Black 194 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Acid Black 194 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Acid Black 194 + H2O2 + H+ | - |
Bacillus subtilis 168 | ? | - |
? | |
| Acid Black 194 + H2O2 + H+ | - |
Pseudomonas putida MET94 | ? | - |
? | |
| Acid Black 210 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Acid Black 210 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Acid Blue 62 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Acid Blue 62 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Acid Orange 7 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Acid Orange 7 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Acid Red 266 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Acid Red 266 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Acid Red 299 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Acid Red 299 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Acid Yellow 49 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Acid Yellow 49 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Direct Blue 1 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Direct Blue 1 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Direct Red 80 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Direct Red 80 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Direct Red R + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Direct Red R + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Disperse Blue 1 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Disperse Blue 1 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Disperse Red 60 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Disperse Red 60 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Fe2+ + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Fe2+ + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| guaiacol + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| guaiacol + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| guaiacol + H2O2 + H+ | - |
Bacillus subtilis 168 | ? | - |
? | |
| guaiacol + H2O2 + H+ | - |
Pseudomonas putida MET94 | ? | - |
? | |
| Methyl Red + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Methyl Red + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Mn2+ + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Mn2+ + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Mordant Black 17 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Mordant Black 17 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Mordant Black 3 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Mordant Black 3 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Mordant Black 9 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Mordant Black 9 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Reactive Blue 19 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Reactive Blue 19 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Reactive Blue 5 + H2O2 | - |
Bacillus subtilis | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
| Reactive Blue 5 + H2O2 | - |
Pseudomonas putida | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
| Reactive Blue 5 + H2O2 | - |
Bacillus subtilis 168 | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
| Reactive Blue 5 + H2O2 | - |
Pseudomonas putida MET94 | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
| Reactive Red 4 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Reactive Red 4 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| Reactive Yellow 145 + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| Reactive Yellow 145 + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| syringaldehyde + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| syringaldehyde + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
| veratryl alcohol + H2O2 + H+ | - |
Bacillus subtilis | ? | - |
? | |
| veratryl alcohol + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 32000, SDS-PAGE | Pseudomonas putida |
| monomer | 1 * 48000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dye-decolorizing peroxidase | - |
Bacillus subtilis |
| dye-decolorizing peroxidase | - |
Pseudomonas putida |
| DyP | - |
Bacillus subtilis |
| DyP | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 30 | - |
Pseudomonas putida |
| 20 | 30 | - |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the enzyme activity remains stable for 53 h at 40°C | Bacillus subtilis |
| 40 | - |
the enzyme activity remains stable for 53 h at 40°C | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Bacillus subtilis |
| 5 | - |
- |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
the enzyme activity remains stable for 16 h at pH 5.0 | Bacillus subtilis |
| 5 | - |
the enzyme activity remains stable for 16 h at pH 5.0 | Pseudomonas putida |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
H2O2 | at pH 4.0 and 30°C | Bacillus subtilis | |
| 0.43 | - |
H2O2 | at pH 5.0 and 30°C | Pseudomonas putida | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
guaiacol | at pH 4.0 and 30°C | Bacillus subtilis | |
| 1.2 | - |
syringaldehyde | at pH 5.0 and 30°C | Pseudomonas putida | |
| 2.4 | - |
acetosyringone | at pH 5.0 and 30°C | Pseudomonas putida | |
| 3.4 | - |
guaiacol | at pH 5.0 and 30°C | Pseudomonas putida | |
| 8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 5.0 and 30°C | Pseudomonas putida | |
| 10 | - |
Mordant black 9 | at pH 4.0 and 30°C | Bacillus subtilis | |
| 20 | - |
Acid Blue 62 | at pH 4.0 and 30°C | Bacillus subtilis | |
| 50 | - |
Mordant black 9 | at pH 5.0 and 30°C | Pseudomonas putida | |
| 50 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Bacillus subtilis | |
| 52 | - |
Mn2+ | at pH 5.0 and 30°C | Pseudomonas putida | |
| 70 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 30°C | Bacillus subtilis | |
| 180 | - |
H2O2 | with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 5.0 and 30°C | Pseudomonas putida | |
| 200 | - |
Reactive Blue 5 | at pH 5.0 and 30°C | Pseudomonas putida | |
| 240 | - |
Acid Blue 62 | at pH 5.0 and 30°C | Pseudomonas putida | |
| 2000 | - |
H2O2 | with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 4.0 and 30°C | Bacillus subtilis | |
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