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Literature summary for 1.11.1.19 extracted from

  • Kimani, V.; Ullrich, R.; Buettner, E.; Herzog, R.; Kellner, H.; Jehmlich, N.; Hofrichter, M.; Liers, C.
    First dye-decolorizing peroxidase from an ascomycetous fungus secreted by Xylaria grammica (2021), Biomolecules, 11, 1391 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 2,6-dimethoxyphenol at pH 4.5 and 25°C Xylaria grammica
0.041
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 25°C Xylaria grammica
0.041
-
 Reactive Blue 5 at pH 4.5 and 25°C Xylaria grammica
0.049
-
 Mn2+ at pH 4.5 and 25°C Xylaria grammica

Organism

Organism UniProt Comment Textmining
Xylaria grammica
-
-
-
Xylaria grammica IHIA82
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Superdex 75 gel filtration, and MonoQ column chromatography Xylaria grammica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
 culture filtrate, at pH 4.5 and 25°C Xylaria grammica
51
-
 after 15fold purification, at pH 4.5 and 25°C Xylaria grammica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Xylaria grammica oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Xylaria grammica IHIA82 oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Xylaria grammica oxidized 2,6-dimethoxyphenol + H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Xylaria grammica IHIA82 oxidized 2,6-dimethoxyphenol + H2O
-
 ?
Mn2+ + H2O2 + H+
-
 Xylaria grammica Mn3+ + 2 H2O
-
 ?
Mn2+ + H2O2 + H+
-
 Xylaria grammica IHIA82 Mn3+ + 2 H2O
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Xylaria grammica oxidized Reactive Blue 5 + H2O
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Xylaria grammica IHIA82 oxidized Reactive Blue 5 + H2O
-
 ?
veratryl alcohol + H2O2 + H+
-
 Xylaria grammica veratraldehyde + H2O
-
 ?
veratryl alcohol + H2O2 + H+
-
 Xylaria grammica IHIA82 veratraldehyde + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Xylaria grammica

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Xylaria grammica
DyP
-
 Xylaria grammica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 Mn2+ at pH 4.5 and 25°C Xylaria grammica
12
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 25°C Xylaria grammica
20
-
 Reactive Blue 5 at pH 4.5 and 25°C Xylaria grammica
29
-
 2,6-dimethoxyphenol at pH 4.5 and 25°C Xylaria grammica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 pH optimum for the oxidation of 2,6-dimethoxyphenol Xylaria grammica
4
-
 pH optimum for the oxidation of Reactive Blue 5 Xylaria grammica
4.5
-
 pH optimum for the oxidation of Mn2+ Xylaria grammica

pH Range

pH Minimum pH Maximum Comment Organism
3 4.5 the oxidation of 2,6-dimethoxyphenol occurs with with approximately 95% and 90% of the maximum activity at pH 3.0 and 4.0, respectively. Above pH 4.0, 2,6-dimethoxyphenol oxidation drops rapidly to 20% at pH 5.0 and to almost zero at pH 6.0. On the other hand, over 40% activity remains at pH 2.0. The oxidation of Reactive Blue 5 occurs with with approximately 50% of the maximum activity at pH 3.0 and 4.5, respectively Xylaria grammica
4 5.5 the curve for the oxidation of Mn2+ shows appreciable activities down to pH 4.0 and up to pH 5.5 (80-90% in relation to the maximal activity). Mn2+ oxidizing activities are still detectable at less optimal pH above 5.5 Xylaria grammica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 Mn2+ at pH 4.5 and 25°C Xylaria grammica
287
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 25°C Xylaria grammica
495
-
 Reactive Blue 5 at pH 4.5 and 25°C Xylaria grammica
2499
-
 2,6-dimethoxyphenol at pH 4.5 and 25°C Xylaria grammica