KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2,6-dimethoxyphenol | at pH 4.5 and 25°C | Xylaria grammica | |
0.041 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.5 and 25°C | Xylaria grammica | |
0.041 | - |
Reactive Blue 5 | at pH 4.5 and 25°C | Xylaria grammica | |
0.049 | - |
Mn2+ | at pH 4.5 and 25°C | Xylaria grammica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xylaria grammica | - |
- |
- |
Xylaria grammica IHIA82 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Superdex 75 gel filtration, and MonoQ column chromatography | Xylaria grammica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.4 | - |
culture filtrate, at pH 4.5 and 25°C | Xylaria grammica |
51 | - |
after 15fold purification, at pH 4.5 and 25°C | Xylaria grammica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Xylaria grammica | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Xylaria grammica IHIA82 | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Xylaria grammica | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Xylaria grammica IHIA82 | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
Mn2+ + H2O2 + H+ | - |
Xylaria grammica | Mn3+ + 2 H2O | - |
? | |
Mn2+ + H2O2 + H+ | - |
Xylaria grammica IHIA82 | Mn3+ + 2 H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | - |
Xylaria grammica | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | - |
Xylaria grammica IHIA82 | oxidized Reactive Blue 5 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Xylaria grammica | veratraldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Xylaria grammica IHIA82 | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Xylaria grammica |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Xylaria grammica |
DyP | - |
Xylaria grammica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
Mn2+ | at pH 4.5 and 25°C | Xylaria grammica | |
12 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.5 and 25°C | Xylaria grammica | |
20 | - |
Reactive Blue 5 | at pH 4.5 and 25°C | Xylaria grammica | |
29 | - |
2,6-dimethoxyphenol | at pH 4.5 and 25°C | Xylaria grammica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
pH optimum for the oxidation of 2,6-dimethoxyphenol | Xylaria grammica |
4 | - |
pH optimum for the oxidation of Reactive Blue 5 | Xylaria grammica |
4.5 | - |
pH optimum for the oxidation of Mn2+ | Xylaria grammica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 4.5 | the oxidation of 2,6-dimethoxyphenol occurs with with approximately 95% and 90% of the maximum activity at pH 3.0 and 4.0, respectively. Above pH 4.0, 2,6-dimethoxyphenol oxidation drops rapidly to 20% at pH 5.0 and to almost zero at pH 6.0. On the other hand, over 40% activity remains at pH 2.0. The oxidation of Reactive Blue 5 occurs with with approximately 50% of the maximum activity at pH 3.0 and 4.5, respectively | Xylaria grammica |
4 | 5.5 | the curve for the oxidation of Mn2+ shows appreciable activities down to pH 4.0 and up to pH 5.5 (80-90% in relation to the maximal activity). Mn2+ oxidizing activities are still detectable at less optimal pH above 5.5 | Xylaria grammica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
Mn2+ | at pH 4.5 and 25°C | Xylaria grammica | |
287 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.5 and 25°C | Xylaria grammica | |
495 | - |
Reactive Blue 5 | at pH 4.5 and 25°C | Xylaria grammica | |
2499 | - |
2,6-dimethoxyphenol | at pH 4.5 and 25°C | Xylaria grammica |