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Literature summary for 1.11.1.19 extracted from

  • Chaplin, A.; Chicano, T.; Hampshire, B.; Wilson, M.; Hough, M.; Svistunenko, D.; Worrall, J.
    An Aromatic dyad motif in dye decolourising peroxidases has implications for free radical formation and catalysis (2019), Chem. Eur. J., 25, 6141-6153 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces lividans

Protein Variants

Protein Variants Comment Organism
Y288F kcat is least affected by the mutation whereas Km shows significant increase compared to the wild type enzyme Streptomyces lividans
Y374F the mutation results in an overall 5.5fold decrease in catalytic efficiency as compared to the wild type enzyme Streptomyces lividans
Y437F kcat is least affected by the mutation whereas Km shows significant increase compared to the wild type enzyme Streptomyces lividans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 25°C Streptomyces lividans
1.3
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y288F, at pH 5.0 and 25°C Streptomyces lividans
1.8
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y437F, at pH 5.0 and 25°C Streptomyces lividans
6.3
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y374F, at pH 5.0 and 25°C Streptomyces lividans

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
Streptomyces lividans oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
?

Synonyms

Synonyms Comment Organism
DtpA
-
Streptomyces lividans
DyP
-
Streptomyces lividans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 25°C Streptomyces lividans
1.3
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y288F, at pH 5.0 and 25°C Streptomyces lividans
1.5
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y437F, at pH 5.0 and 25°C Streptomyces lividans
1.8
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme Y374F, at pH 5.0 and 25°C Streptomyces lividans

Cofactor

Cofactor Comment Organism Structure
heme
-
Streptomyces lividans