Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Streptomyces lividans |
Protein Variants | Comment | Organism |
---|---|---|
Y288F | kcat is least affected by the mutation whereas Km shows significant increase compared to the wild type enzyme | Streptomyces lividans |
Y374F | the mutation results in an overall 5.5fold decrease in catalytic efficiency as compared to the wild type enzyme | Streptomyces lividans |
Y437F | kcat is least affected by the mutation whereas Km shows significant increase compared to the wild type enzyme | Streptomyces lividans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 5.0 and 25°C | Streptomyces lividans | |
1.3 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme Y288F, at pH 5.0 and 25°C | Streptomyces lividans | |
1.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme Y437F, at pH 5.0 and 25°C | Streptomyces lividans | |
6.3 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme Y374F, at pH 5.0 and 25°C | Streptomyces lividans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Streptomyces lividans | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DtpA | - |
Streptomyces lividans |
DyP | - |
Streptomyces lividans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 5.0 and 25°C | Streptomyces lividans | |
1.3 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme Y288F, at pH 5.0 and 25°C | Streptomyces lividans | |
1.5 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme Y437F, at pH 5.0 and 25°C | Streptomyces lividans | |
1.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme Y374F, at pH 5.0 and 25°C | Streptomyces lividans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Streptomyces lividans |