Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-arbutin | 24.2% inhibition at 1.0 mM, 66.4% inhibition at 50 mM | Streptomyces albidoflavus | |
acetonitrile | less than 5% residual activity at 40% (v/v) | Streptomyces albidoflavus | |
ascorbate | 39.5% inhibition at 0.05 mM, complete inhibition at 0.5 mM | Streptomyces albidoflavus | |
azide | 26.6% inhibition at 5 mM, 68.4% inhibition at 50 mM | Streptomyces albidoflavus | |
DMSO | less than 10% residual activity at 40% (v/v) | Streptomyces albidoflavus | |
ethanol | about 30% residual activity at 40% (v/v) | Streptomyces albidoflavus | |
H2O2 | 42% inhibition at 0.01 mM, complete inhibition at 0.05 mM | Streptomyces albidoflavus | |
L-cysteine | 33.9% inhibition at 0.02 mM, complete inhibition at 0.1 mM | Streptomyces albidoflavus | |
Metabisulfite | 93.5% inhibition at 0.5 mM, complete inhibition at 1.0 mM | Streptomyces albidoflavus | |
methanol | about 40% residual activity at 40% (v/v) | Streptomyces albidoflavus | |
additional information | not inhibited by EDTA, SDS, and NaCl | Streptomyces albidoflavus | |
Propanol | less than 40% residual activity at 30% (v/v) | Streptomyces albidoflavus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
2,4-Dichlorophenol | at pH 8.0 and 25°C | Streptomyces albidoflavus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | about 170% activity at 1 mM | Streptomyces albidoflavus | |
additional information | not affected by Ca2+, Co2+, Fe2+, Mg2+, Mn2+, and Zn2+ | Streptomyces albidoflavus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces albidoflavus | - |
- |
- |
Streptomyces albidoflavus BSII#1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation and ultrafiltration | Streptomyces albidoflavus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46.51 | - |
after 46.51fold purification, at pH 8.0 and 25°C | Streptomyces albidoflavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,4-dichlorophenol + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized 2,4-dichlorophenol + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces albidoflavus BSII#1 | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
2-aminophenol + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized 2-aminophenol + H2O | - |
? | |
Azure B + H2O2 + H+ | approximately 30% decolourisation is observed after 24 h (0.001 mM dye) | Streptomyces albidoflavus | oxidized Azure B + H2O | - |
? | |
caffeic acid + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized caffeic acid + H2O | - |
? | |
catechol + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized catechol + H2O | - |
? | |
guaiacol + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized guaiacol + H2O | - |
? | |
guaiacol + H2O2 + H+ | - |
Streptomyces albidoflavus BSII#1 | oxidized guaiacol + H2O | - |
? | |
L-DOPA + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized L-DOPA + H2O | - |
? | |
additional information | no activity with 3-hydroxyanthranilic acid, 4-methylcatechol, L-tyrosine, 4-cresol, resorcinol, and syringaldazine | Streptomyces albidoflavus | ? | - |
- |
|
additional information | the enzyme also successfully catalyses coupling reactions between several phenolic monomer pairs including catechin-caffeic acid, catechin-catechol, catechin-guaiacol and guaiacol-syringaldazine | Streptomyces albidoflavus | ? | - |
- |
|
additional information | no activity with 3-hydroxyanthranilic acid, 4-methylcatechol, L-tyrosine, 4-cresol, resorcinol, and syringaldazine | Streptomyces albidoflavus BSII#1 | ? | - |
- |
|
additional information | the enzyme also successfully catalyses coupling reactions between several phenolic monomer pairs including catechin-caffeic acid, catechin-catechol, catechin-guaiacol and guaiacol-syringaldazine | Streptomyces albidoflavus BSII#1 | ? | - |
- |
|
phenol + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized phenol + H2O | - |
? | |
pyrogallol + H2O2 + H+ | - |
Streptomyces albidoflavus | oxidized pyrogallol + H2O | - |
? | |
Reactive Black 5 + H2O2 + H+ | approximately 30% decolourisation is observed after 24 h (0.001 mM dye) | Streptomyces albidoflavus | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Black 5 + H2O2 + H+ | approximately 30% decolourisation is observed after 24 h (0.001 mM dye) | Streptomyces albidoflavus BSII#1 | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Blue 4 + H2O2 + H+ | approximately 30% decolourisation is observed after 24 h (0.001 mM dye) | Streptomyces albidoflavus | oxidized Reactive Blue 4 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 46000, SDS-PAGE | Streptomyces albidoflavus |
homodimer | 2 * 45390, calculated from amino acid sequence | Streptomyces albidoflavus |
Synonyms | Comment | Organism |
---|---|---|
DyP | - |
Streptomyces albidoflavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces albidoflavus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 80 | the enzyme is active over a wide temperature range (from 5 to 80°C) | Streptomyces albidoflavus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | the enzyme remains stable at temperatures of up to 80°C for 60 min | Streptomyces albidoflavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
the enzyme has two pH optima at 3.0 and 8.0, depending on substrate | Streptomyces albidoflavus |
8 | - |
the enzyme has two pH optima at 3.0 and 8.0, depending on substrate | Streptomyces albidoflavus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | more than 50% activity after 24 h between pH 5.0-9.0, total loss of activity after the enzyme is incubated at pH 3.0-4.0, and more than 90% loss of activity at pH 4.5 | Streptomyces albidoflavus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Streptomyces albidoflavus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces albidoflavus | calculated from amino acid sequence | - |
6.7 |