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Literature summary for 1.11.1.19 extracted from

  • Guo, W.; Xu, J.; Wu, S.; Gao, S.; Wen, G.; Tan, X.; Lin, Y.
    Design and engineering of an efficient peroxidase using myoglobin for dye decolorization and lignin bioconversion (2022), Int. J. Mol. Sci., 23, 413 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Physeter catodon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 7.0 and 25°C Physeter catodon
0.11
-
 guaiacol at pH 7.0 and 25°C Physeter catodon
5.5
-
 H2O2 at pH 7.0 and 25°C Physeter catodon

Organism

Organism UniProt Comment Textmining
Physeter catodon
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Physeter catodon oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
Amaranth + H2O2 + H+ 82% decolorization after 30 min Physeter catodon oxidized Amaranth + H2O
-
 ?
Brilliant Blue R + H2O2 + H+ 54% decolorization after 1 h Physeter catodon oxidized Brilliant Blue R + H2O
-
 ?
guaiacol + H2O2 + H+
-
 Physeter catodon oxidized guaiacol + H2O
-
 ?
guaiacylglycerol-beta-guaiacyl ether + H2O2 + H+
-
 Physeter catodon oxidized guaiacylglycerol-beta-guaiacyl ether + H2O
-
 ?
Kraft lignin + H2O2 + H+
-
 Physeter catodon oxidized Kraft lignin + H2O
-
 ?
Malachite Green + H2O2 + H+ 94% decolorization after 1 h Physeter catodon oxidized Malachite Green + H2O
-
 ?
Reactive Black 5 + H2O2 + H+ 54% decolorization after 30 min Physeter catodon oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 19 + H2O2 + H+ 85% decolorization after less than 10 s Physeter catodon oxidized Reactive Blue 19 + H2O
-
 ?
Reactive Orange 16 + H2O2 + H+ 70% decolorization after 30 min Physeter catodon oxidized Reactive Orange 16 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DyP
-
 Physeter catodon
YRW2 Mb myoglobin mutant F43Y/T67R/P88W/F138W exhibiting considerably enhanced dye decolorizing peroxidase activity Physeter catodon

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 guaiacol at pH 7.0 and 25°C Physeter catodon
40.6
-
 H2O2 at pH 7.0 and 25°C Physeter catodon
76.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 7.0 and 25°C Physeter catodon

Cofactor

Cofactor Comment Organism Structure
heme
-
 Physeter catodon

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
103.4
-
 guaiacol at pH 7.0 and 25°C Physeter catodon
1276.57
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 7.0 and 25°C Physeter catodon