Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain UM262 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
R463L | the mutation is found in isoniazid-resistant strains and does not lead to a loss of peroxidase or catalase activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.18 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis | |
6.16 | - |
H2O2 | mutant enzyme R463L, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
2 * 82000, SDS-PAGE | Mycobacterium tuberculosis |
160000 | - |
Superose 12HR gel filtration | Mycobacterium tuberculosis |
175000 | - |
Sephadex G200 gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Mono Q column chromatography, DEAE-Sephacel gel filtration, and Superdex S200 gel filtration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00904 | - |
specific peroxidase activity, using isoniazid as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
0.0098 | - |
specific peroxidase activity, using isoniazid as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
0.208 | - |
specific peroxidase activity, using NADH as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
0.212 | - |
specific peroxidase activity, using NADH as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
0.264 | - |
specific peroxidase activity, using NADPH as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
0.272 | - |
specific peroxidase activity, using NADPH as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
2.12 | - |
specific peroxidase activity, using pyrogallol as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
2.29 | - |
specific peroxidase activity, using pyrogallol as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
22.8 | - |
specific peroxidase activity, using o-dianisidine as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
25.9 | - |
specific peroxidase activity, using o-dianisidine as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
99.4 | - |
specific peroxidase activity, using 4-phenylendiamine as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
100.7 | - |
specific peroxidase activity, using 4-phenylendiamine as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C | Mycobacterium tuberculosis |
226 | - |
specific catalase activity, crude extract, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis |
2420 | - |
specific catalase activity, after 10.7fold purification, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-80°C, 20 mM MOPS, pH 7.5, containing 40% (v/v) glycerol, 6 months, no loss of activity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-phenylenediamine + H2O2 | highest specific peroxidase activity | Mycobacterium tuberculosis | oxidized 4-phenylenediamine + H2O | - |
? | |
H2O2 | - |
Mycobacterium tuberculosis | O2 + H2O | - |
? | |
isoniazid + H2O2 | i.e. isonicotinic acid hydrazide | Mycobacterium tuberculosis | oxidized isoniazid + H2O | - |
? | |
additional information | the wild type enzyme functions as a highly active catalase as well as a broad specificity peroxidase | Mycobacterium tuberculosis | ? | - |
? | |
NADH + H2O2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
NADPH + H2O2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
o-dianisidine + H2O2 | - |
Mycobacterium tuberculosis | oxidized o-dianisidine + H2O | - |
? | |
pyrogallol + H2O2 | - |
Mycobacterium tuberculosis | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 82000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10100 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis | |
11400 | - |
H2O2 | mutant enzyme R463L, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
for catalase activity | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the heme content of the enzyme is calculated to be 0.98 heme per dimer | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | oxidation of isoniazid by KatG in the presence of InhA leads to the inactivation of InhA | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1850 | - |
H2O2 | mutant enzyme R463L, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis | |
1950 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C | Mycobacterium tuberculosis |