Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain UM262 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
A110V | site-directed mutagenesis, catalytic efficiency with and activation of isoniazid similar to the wild-type enzyme, reduced Km and increased kcat compared to the wild-type | Mycobacterium tuberculosis |
A139P | site-directed mutagenesis, 76% decreased activity with and activation of isoniazid compared to the wild-type enzyme | Mycobacterium tuberculosis |
D735A | site-directed mutagenesis, 73% decreased activity with and activation of isoniazid compared to the wild-type enzyme | Mycobacterium tuberculosis |
L619P | site-directed mutagenesis, no remaining activity with and activation of isoniazid | Mycobacterium tuberculosis |
L634F | site-directed mutagenesis, 36% decreased activity with and activation of isoniazid compared to the wild-type enzyme | Mycobacterium tuberculosis |
S315N | site-directed mutagenesis, no remaining activity with and activation of isoniazid | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
2-dianisidine | recombinant mutant D735A, 23°C | Mycobacterium tuberculosis | |
0.064 | - |
2-dianisidine | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis | |
0.065 | - |
2-dianisidine | recombinant mutant L634F, 23°C | Mycobacterium tuberculosis | |
0.078 | - |
2-dianisidine | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis | |
0.081 | - |
2-dianisidine | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
0.084 | - |
2-dianisidine | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
0.096 | - |
2-dianisidine | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
5.9 | - |
H2O2 | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
10.3 | - |
H2O2 | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis | |
10.7 | - |
H2O2 | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
14.3 | - |
H2O2 | recombinant mutant L634F, 23°C | Mycobacterium tuberculosis | |
14.8 | - |
H2O2 | recombinant mutant D735A, 23°C | Mycobacterium tuberculosis | |
16.8 | - |
H2O2 | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
17.1 | - |
H2O2 | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates isoniazid activation, not essential | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Mycobacterium tuberculosis | - |
O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
enzyme KatG, gene katG | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain UM262, 50-90% homogeneity | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isoniazid activation activities of wild-type and mutant enzymes in absence or presence of Mn2+, overview | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dianisidine + tert-butyl hydroperoxide | peroxidase activity | Mycobacterium tuberculosis | ? | - |
? | |
H2O2 | - |
Mycobacterium tuberculosis | O2 + H2O | - |
? | |
H2O2 | catalase activity | Mycobacterium tuberculosis | O2 + H2O | - |
? | |
isoniazid + NADH | isoniazid activation | Mycobacterium tuberculosis | isonicotinic acyl-NADH | the product is a strong inhibitor of the NADH-dependent enoyl-[acyl carrier protein] reductase InhA and the beta-ketoacyl [acyl carrier protein] synthase | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
catalase-peroxidase | - |
Mycobacterium tuberculosis |
KatG | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.86 | - |
2-dianisidine | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
0.99 | - |
2-dianisidine | recombinant mutants L634F and D735A, 23°C | Mycobacterium tuberculosis | |
1.06 | - |
2-dianisidine | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis | |
1.69 | - |
2-dianisidine | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
3.48 | - |
2-dianisidine | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
4.85 | - |
2-dianisidine | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis | |
2750 | - |
H2O2 | recombinant mutant D735A, 23°C | Mycobacterium tuberculosis | |
2990 | - |
H2O2 | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
3180 | - |
H2O2 | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis | |
3270 | - |
H2O2 | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
3330 | - |
H2O2 | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
3450 | - |
H2O2 | recombinant mutant L634F, 23°C | Mycobacterium tuberculosis | |
5230 | - |
H2O2 | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | 1.2-1.5 hemes per enzyme dimer | Mycobacterium tuberculosis |