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Literature summary for 1.11.1.21 extracted from

  • Calandrelli, V.; Gambacorta, A.; Romano, I.; Carratore, V.; Lama, L.
    A novel thermo-alkali stable catalase-peroxidase from Oceanobacillus oncorhynchi subsp. incaldaniensis: Purification and characterization (2008), World J. Microbiol. Biotechnol., 24, 2269-2275.
No PubMed abstract available
Search for more literature for 1.11.1.21

Activating Compound

Activating Compound Comment Organism Structure
NaCl 76% stimulation of catalase by addition of 10 mM NaCl Oceanobacillus oncorhynchi subsp. incaldanensis

Inhibitors

Inhibitors Comment Organism Structure
azide strong inhibition Oceanobacillus oncorhynchi subsp. incaldanensis
Ba2+ partial inhibition of catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
Co2+ partial inhibition of catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
cyanide
-
 Oceanobacillus oncorhynchi subsp. incaldanensis
EDTA 27% inhibition at 10 mM Oceanobacillus oncorhynchi subsp. incaldanensis
H2O2 17% inhibition at 80 mM Oceanobacillus oncorhynchi subsp. incaldanensis
histidine 19% inhibition at 10 mM Oceanobacillus oncorhynchi subsp. incaldanensis
hydroxylamine strong inhibition Oceanobacillus oncorhynchi subsp. incaldanensis
KCl 50% inhibition at 20 mM Oceanobacillus oncorhynchi subsp. incaldanensis
MgCl2 50% inhibition at 10 mM Oceanobacillus oncorhynchi subsp. incaldanensis
additional information the enzyme is insensitive to 10 mM 3-amino-1,2,4-triazole Oceanobacillus oncorhynchi subsp. incaldanensis
NaCl 50% inhibition at 5 mM Oceanobacillus oncorhynchi subsp. incaldanensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24
-
 H2O2 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oceanobacillus oncorhynchi subsp. incaldanensis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron is present at 1 atom per homodimer Oceanobacillus oncorhynchi subsp. incaldanensis
K+ 2 mM K+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
Mg2+ 2 mM Mg2+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
Mn2+ 2 mM Mn2+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
Na+ 2 mM Na+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi subsp. incaldanensis
280000
-
 gel filtration Oceanobacillus oncorhynchi subsp. incaldanensis

Organism

Organism UniProt Comment Textmining
Oceanobacillus oncorhynchi subsp. incaldanensis
-
-
-
Oceanobacillus oncorhynchi subsp. incaldanensis 20AG
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Oceanobacillus oncorhynchi subsp. incaldanensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
168
-
 unpurified cytosolic fraction, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis
316
-
 unpurified cytosolic fraction, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis
7512
-
 after 44.7fold purification, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis
35000
-
 after 110.7fold purification, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminoantipyrine + H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis oxidized 4-aminoantipyrine + H2O
-
 ?
4-aminoantipyrine + H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis 20AG oxidized 4-aminoantipyrine + H2O
-
 ?
H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis O2 + H2O
-
 ?
H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis 20AG O2 + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi subsp. incaldanensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the catalatic activity of the enzyme is stable for 24 h at 40°C. At 50°C the half life is about 18 h, while at 60°C over the first 4 h of incubation 50% of activity is observed Oceanobacillus oncorhynchi subsp. incaldanensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

pH Range

pH Minimum pH Maximum Comment Organism
5 10 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 the catalatic activity of the enzyme is stable in the pH range of 5.0 to 10.0 after 1 h of treatment at 40°C Oceanobacillus oncorhynchi subsp. incaldanensis

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains 1 heme per homodimer Oceanobacillus oncorhynchi subsp. incaldanensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis NaCl
10
-
 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis MgCl2
20
-
 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis KCl