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Literature summary for 1.11.1.21 extracted from
- Purification and characterization of a catalase-peroxidase and a typical catalase from the bacterium Klebsiella pneumoniae (1991), Biochim. Biophys. Acta, 1077, 299-307.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Klebsiella pneumoniae |  |
| cyanide | - |
Klebsiella pneumoniae | |
| additional information | the enzyme is not inhibited by 3-amino-1,2,4-triazole | Klebsiella pneumoniae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
H2O2 | peroxidase activity, pH 5.3, 30°C | Klebsiella pneumoniae | |
| 6.5 | - |
H2O2 | catalase activity, pH 6.3, 30°C | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62500 | - |
4 * 62500, estimated from SDS-PAGE | Klebsiella pneumoniae |
| 250000 | - |
gel filtration | Klebsiella pneumoniae |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | 60 min incubation with 4 M urea causes 72% inhibition of the enzyme activity | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
| Klebsiella pneumoniae KP1 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| incubation of the enzyme for 80 min with 2 mM H2O2 causes 88% inhibition, the enzyme is completely inhibited after 50 min in 10 mM H2O2, the enzyme retains 5% of its activity after 10 min in 50 mM H2O2 | Klebsiella pneumoniae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl-Sepharose column chromatography, Sepharose column chromatography, and DEAE-cellulose chromatography | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43 | - |
crude enzyme, in 100 mM potassium phosphate buffer (pH 6.8), at 30°C | Klebsiella pneumoniae |
| 560 | - |
after 13fold purification, in 100 mM potassium phosphate buffer (pH 6.8), at 30°C | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Klebsiella pneumoniae | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
| diaminobenzidine + H2O2 | - |
Klebsiella pneumoniae | oxidized diaminobenzidine + H2O | - |
? | |
| H2O2 | - |
Klebsiella pneumoniae | O2 + H2O | - |
? | |
| H2O2 | - |
Klebsiella pneumoniae KP1 | O2 + H2O | - |
? | |
| additional information | guiacol and ascorbate are no substrates | Klebsiella pneumoniae | ? | - |
? | |
| additional information | guiacol and ascorbate are no substrates | Klebsiella pneumoniae KP1 | ? | - |
? | |
| NADH + H2O2 | - |
Klebsiella pneumoniae | ? | - |
? | |
| NADH + H2O2 | - |
Klebsiella pneumoniae KP1 | ? | - |
? | |
| NADPH + H2O2 | - |
Klebsiella pneumoniae | ? | - |
? | |
| o-dianisidine + H2O2 | - |
Klebsiella pneumoniae | oxidized o-dianisidine + H2O | - |
? | |
| o-dianisidine + H2O2 | - |
Klebsiella pneumoniae KP1 | oxidized o-dianisidine + H2O | - |
? | |
| pyrogallol + H2O2 | - |
Klebsiella pneumoniae | ? + H2O | - |
? | |
| pyrogallol + H2O2 | - |
Klebsiella pneumoniae KP1 | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 62500, estimated from SDS-PAGE | Klebsiella pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KpCP | - |
Klebsiella pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 51 | the enzyme is stable for 30 min up to 30°C and loses 50% of its activity at 43°C, 10 min at 51°C result in complete inactivation of the enzyme | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 5.7 | peroxidatic activity | Klebsiella pneumoniae |
| 6.3 | - |
catalatic activity | Klebsiella pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | peroxidatic activity | Klebsiella pneumoniae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 10.8 | the enzyme is stable for 30 min in the pH range of 6.2-8.1, incubation of the enzyme for 30 min at pH 5.2 and 10.8 causes 50% inhibition | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Klebsiella pneumoniae | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
pH 6.3, at 30°C | Klebsiella pneumoniae | azide | |
| 0.035 | - |
pH 6.3, at 30°C | Klebsiella pneumoniae | cyanide | |
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